Membrane properties of hydroxycholesterols related to the brain cholesterol metabolism.

Beilstein Journal of Organic Chemistry
Malte HilschHolger A Scheidt

Abstract

Compared to cholesterol, hydroxycholesterols contain an additional hydroxy group in the alkyl chain and are able to efficiently cross the brain-blood barrier. Therefore, they are responsible for the sterol transfer between brain and circulation. The current study compares the membrane properties of several hydroxycholesterols with those of cholesterol using 2H NMR spectroscopy, a membrane permeability assay, and fluorescence microscopy experiments. It is shown that hydroxycholesterols do not exert the unique impact on membrane properties characteristic for cholesterol with regard to the influence on lipid chain order, membrane permeability and formation of lateral domains.

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Citations

Apr 24, 2018·Frontiers in Cell and Developmental Biology·Howard R Petty
Nov 3, 2020·Chemistry and Physics of Lipids·Maria SzomekDaniel Wüstner

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Methods Mentioned

BETA
NMR
fluorescence
fluorescence microscopy

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