Membrane protein MHZ3 stabilizes OsEIN2 in rice by interacting with its Nramp-like domain

Proceedings of the National Academy of Sciences of the United States of America
Biao MaJin-Song Zhang

Abstract

The phytohormone ethylene regulates many aspects of plant growth and development. EIN2 is the central regulator of ethylene signaling, and its turnover is crucial for triggering ethylene responses. Here, we identified a stabilizer of OsEIN2 through analysis of the rice ethylene-response mutant mhz3. Loss-of-function mutations lead to ethylene insensitivity in etiolated rice seedlings. MHZ3 encodes a previously uncharacterized membrane protein localized to the endoplasmic reticulum. Ethylene induces MHZ3 gene and protein expression. Genetically, MHZ3 acts at the OsEIN2 level in the signaling pathway. MHZ3 physically interacts with OsEIN2, and both the N- and C-termini of MHZ3 specifically associate with the OsEIN2 Nramp-like domain. Loss of mhz3 function reduces OsEIN2 abundance and attenuates ethylene-induced OsEIN2 accumulation, whereas MHZ3 overexpression elevates the abundance of both wild-type and mutated OsEIN2 proteins, suggesting that MHZ3 is required for proper accumulation of OsEIN2 protein. The association of MHZ3 with the Nramp-like domain is crucial for OsEIN2 accumulation, demonstrating the significance of the OsEIN2 transmembrane domains in ethylene signaling. Moreover, MHZ3 negatively modulates OsEIN2 ubiquitinat...Continue Reading

References

Sep 22, 1995·Science·J HuaE M Meyerowitz
Mar 28, 2002·The Journal of Biological Chemistry·Yi-Feng ChenG Eric Schaller
Apr 6, 2002·Science·Stephen A GoffSteven Briggs
Nov 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chuanli JuCaren Chang
Mar 21, 2013·The Plant Cell·Gyeong Mee Yoon, Joseph J Kieber
Mar 4, 2015·Molecular Plant·Chao YangJin-Song Zhang
Aug 8, 2015·Plant Physiology·Chuanli Ju, Caren Chang
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Fan ZhangHong Qiao

❮ Previous
Next ❯

Citations

Jul 30, 2019·Frontiers in Plant Science·Hua QinRongfeng Huang
Mar 5, 2020·Journal of Integrative Plant Biology·Fanwei MengJun Liu
Nov 23, 2018·International Journal of Molecular Sciences·Hua Qin, Rongfeng Huang
Dec 20, 2019·Journal of Experimental Botany·Tegan Armarego-MarriottŁucja Kowalewska
Apr 26, 2020·The Journal of Biological Chemistry·Brad M Binder
Oct 24, 2020·Journal of Integrative Plant Biology·He ZhaoJin-Song Zhang
Jul 30, 2021·Frontiers in Plant Science·Ke ChenMariusz Jaremko

❮ Previous
Next ❯

Methods Mentioned

BETA
histone acetylation
ubiquitination
PCR
RNA-seq
two-hybrid
coimmunoprecipitation
transgenic
Y2H
Co-IP
nuclear translocation

Software Mentioned

InterPro Domain Scan
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.