PMID: 3768359Nov 6, 1986Paper

Membrane protein phosphorylation during stomatocyte-echinocyte transformation of human erythrocytes

Biochimica Et Biophysica Acta
W H ReinhartS Chien

Abstract

The normal, discoid shape of red blood cells represents an equilibrium between two opposing factors, i.e., stomatocytic and echinocytic transformations. Most stomatocytic agents were found to be inhibitors of calmodulin, a regulator of the phosphorylation of membrane proteins. We determined whether red cell shape transformations could be caused by changes in phosphorylation of membrane proteins, specifically the cAMP-dependent phosphorylation of ankyrin and band 4.1. Red blood cells were incubated with 32P and 100 microM chlorpromazine (stomatocytic transformation) or 30 mM sodium salicylate (echinocytic transformation) for various time intervals. Ghost membrane proteins were examined by polyacrylamide gel electrophoresis and autoradiography. Spectrin (beta-chain), ankyrin, band 3, band 4.1 and 4.9 were phosphorylated. No change was found in the degree and pattern of phosphorylation after stomatocytic transformation. Salicylate caused a reversible inhibition of transmembranous phosphate transport in both directions. The results indicate that the stomatocytic transformation induced by chlorpromazine and the echinocytic transformation induced by salicylate do not involve a change in phosphorylation, but that the echinocytic trans...Continue Reading

References

Jan 1, 1978·Journal of Supramolecular Structure·G FairbanksV P Patel
Nov 16, 1973·Biochimica Et Biophysica Acta·G H Bond, D L Clough
Feb 6, 1970·Biochemical and Biophysical Research Communications·W Y Cheung
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M P Sheetz, S J Singer
Jan 1, 1980·Annals of the New York Academy of Sciences·B WeissT L Wallace
Jan 1, 1980·Annals of the New York Academy of Sciences·M VolpiM B Feinstein
Jul 30, 1981·Biochemical and Biophysical Research Communications·T Tanaka, H Hidaka
Nov 20, 1981·Biochimica Et Biophysica Acta·S Thompson, A H Maddy
Mar 1, 1983·The American Journal of Physiology·S R Goodman, K Shiffer
Apr 7, 1982·Biochimica Et Biophysica Acta·M J NelsonW H Huestis
Mar 1, 1983·The Journal of Cell Biology·G A NelsonM J Karnovsky
Jan 1, 1981·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·M C Lecomte, P Boivin
Feb 1, 1981·The Journal of Cell Biology·V P Patel, G Fairbanks
Sep 29, 2011·Applied and Environmental Microbiology·Emiley A EloeDouglas H Bartlett

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