PMID: 701358Sep 1, 1978Paper

Membrane proteins synthesized but not processed by isolated maize chloroplasts

The Journal of Cell Biology
A E GrebanierL Bogorad

Abstract

One-dimensional maps of proteolytic fragments generated by digestion with Staphylococcus aureus protease in sodium dodecyl sulfate (SDS) were used to identify three polypeptides synthesized by isolated Zea mays chloroplasts. This technique does not depend upon proper incorporation of the newly synthesized polypeptides into a more complex structure for their identification. The only preliminary purification required is electrophoretic separation on SDS-polyacrylamide gels. The pattern of radioactive fragments from labeled proteins which co-migrate with the alpha and beta subunits of chloroplast coupling factor (CF1) corresponds precisely to the pattern of stainable fragments derived from subunits of the purified enzyme. A 34,500-dalton protein is the major membrane-associated product of protein synthesis by isolated maize chloroplasts. From the similarity in the fragments formed by digestion with S. aureus protease, it appears that this radioactive protein is probably a precursor of a 32,000-dalton protein which is a component of the thylakoid. The alpha and beta subunits of CF1 newly synthesized by isolated chloroplasts are not fully extractable by procedures which normally solubilize the enzyme from membranes. The 34,500-dalto...Continue Reading

References

Jan 6, 1975·Biochimica Et Biophysica Acta·K W Joy, R J Ellis
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Feb 1, 1976·FEBS Letters·L R Mendiola-MorgenthalerC A Price
Jan 1, 1976·Archives of Biochemistry and Biophysics·J J Morgenthaler, L Mendiola-Morgenthaler
Aug 31, 1973·Biochimica Et Biophysica Acta·H StrotmannK Edelmann
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Nov 1, 1972·The Journal of Cell Biology·A L Hubbard, Z A Cohn

❮ Previous
Next ❯

Citations

Sep 1, 1981·Photosynthesis Research·H P MichelA Boschetti
Jul 1, 1988·Plant Molecular Biology·P GoloubinoffM Edelman
Jul 1, 1988·Photosynthesis Research·J E Hughes, G Link
Jan 1, 1988·Photosynthesis Research·A GnanamR M Mannan
Mar 1, 1985·Plant Molecular Biology·N ChuK Tewari
Jun 7, 2007·Photosynthesis Research·Kimiyuki Satoh, Yumiko Yamamoto
Aug 19, 2008·Photosynthesis Research·Marvin Edelman, Autar K Mattoo
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G H Kidd, L Bogorad
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N NelsonG Schatz
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K E SteinbackC J Arntzen
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K PfisterC J Arntzen
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Watanabe, C A Price
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C J HoweJ C Gray
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B MulliganL Bogorad
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A K Mattoo, M Edelman
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D ZaitlinL Bogorad
May 1, 1984·Plant Physiology·E M Reardon, C A Price
Aug 1, 1984·Plant Physiology·H T Nivison, A T Jagendorf
Jul 1, 1979·European Journal of Biochemistry·A Doherty, J C Gray
Nov 1, 1980·European Journal of Biochemistry·J C Gray
Dec 1, 1991·The American Review of Respiratory Disease·G T AndersH M Blanton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.