Membrane receptors, involved in up-regulation of calcium channels in bovine adrenal chromaffin cells, chronically exposed to ethanol.

Neuropharmacology
C H BrennanJohn M Littleton

Abstract

Release of catecholamines, induced by carbachol, from bovine adrenal chromaffin cells, maintained in culture, is potently inhibited by the presence of ethanol (50 mM). This inhibition is prevented by the concomitant presence of bicuculline (1 microM) or by the inverse agonist at the benzodiazepine receptor, Ro 15 4513 (50 nM), arguing that the effect of ethanol is, at least, partly due to potentiation of endogenous GABA at the GABAA receptor sites on these cells. Exposing cells to medium containing ethanol (200 mM) for 4 days results in an approximately 100% increase in binding sites for [3H]dihydropyridine on membranes of adrenal chromaffin cells. This increase in binding sites was largely prevented by the presence in the culture medium of Ro 15 4513 (50 nM), the dihydropyridine calcium channel agonist BAY K 8644 (50 nM) or the calcium channel antagonist, nitrendipine (50 nM). The results strongly suggest that the increase in binding sites for [3H]dihydropyridine represents an adaptation of cells to overcome the inhibitory effect of ethanol on the excitability of cells (which is, at least partly, due to some action at the GABAA receptor). The protein containing the receptor site for dihydropyridine drugs is clearly also involv...Continue Reading

Citations

Apr 6, 2013·Pflügers Archiv : European journal of physiology·Lynda Sharrett-FieldMark A Prendergast
May 1, 1995·Drug and Alcohol Dependence·H J Little
Jan 1, 1991·Annals of the New York Academy of Sciences·J M LittletonO Bouchenafa
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Jun 28, 1992·Annals of the New York Academy of Sciences·J LittletonR Laverty
May 18, 2005·Alcoholism, Clinical and Experimental Research·Patrick J MulhollandMark A Prendergast
Aug 11, 2015·ACS Applied Materials & Interfaces·Binglin SuiKevin D Belfield
Jul 28, 2020·Analytical and Bioanalytical Chemistry·Juewei NingYanqing Tian

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