Membrane Remodeling as a Key Player of the Hepatotoxicity Induced by Co-Exposure to Benzo[a]pyrene and Ethanol of Obese Zebrafish Larvae

Biomolecules
Muhammad ImranNormand Podechard

Abstract

The rise in prevalence of non-alcoholic fatty liver disease (NAFLD) constitutes an important public health concern worldwide. Including obesity, numerous risk factors of NAFLD such as benzo[a]pyrene (B[a]P) and ethanol have been identified as modifying the physicochemical properties of the plasma membrane in vitro thus causing membrane remodeling-changes in membrane fluidity and lipid-raft characteristics. In this study, the possible involvement of membrane remodeling in the in vivo progression of steatosis to a steatohepatitis-like state upon co-exposure to B[a]P and ethanol was tested in obese zebrafish larvae. Larvae bearing steatosis as the result of a high-fat diet were exposed to ethanol and/or B[a]P for seven days at low concentrations coherent with human exposure in order to elicit hepatotoxicity. In this condition, the toxicant co-exposure raised global membrane order with higher lipid-raft clustering in the plasma membrane of liver cells, as evaluated by staining with the fluoroprobe di-4-ANEPPDHQ. Involvement of this membrane's remodeling was finally explored by using the lipid-raft disruptor pravastatin that counteracted the effects of toxicant co-exposure both on membrane remodeling and toxicity. Overall, it can be...Continue Reading

References

Sep 12, 2000·Current Gastroenterology Reports·Z M Younossi
Dec 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Esther MarzaPatrick J Babin
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marie-Anne RobinBernard Fromenty
Feb 4, 2006·Journal of Biomedical Science·Aseervatham Anusha AmaliJen-Leih Wu
Oct 28, 2006·Alcoholism, Clinical and Experimental Research·Benita L McVickerCarol A Casey
Nov 27, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philippe NourissatOdile Sergent
Oct 17, 2008·Pharmacological Reviews·Nora Anderson, Jürgen Borlak
Jan 8, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael J PasseriKirsten C Sadler
Aug 21, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jaime Chu, Kirsten C Sadler
Nov 26, 2009·Toxicology and Applied Pharmacology·Xavier TekpliDominique Lagadic-Gossmann
Jun 10, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·W AldertonI Gardner
Jul 14, 2010·Journal of Hepatology·Stephen CaldwellJan A Redick
Apr 6, 2011·Alcoholism, Clinical and Experimental Research·Sayaka InokuchiEkihiro Seki
Jun 3, 2011·World Journal of Gastroenterology : WJG·Angela Dolganiuc
Jun 21, 2011·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Waleed Samy, Mohammed A Hassanian
Jul 5, 2011·Journal of Lipid Research·Angèle Tingaud-SequeiraPatrick J Babin
Jul 16, 2011·Recent Patents on Anti-cancer Drug Discovery·Xavier TekpliDominique Lagadic-Gossmann
Oct 12, 2011·Current Opinion in Lipidology·Giovanni MussoRoberto Gambino
Dec 14, 2011·Nature Protocols·Dylan M OwenKatharina Gaus
Dec 20, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P W WesterE D Kroese
Jan 24, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Hideyuki HyogoKazuaki Chayama
May 23, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xavier TekpliDominique Lagadic-Gossmann
Jun 9, 2012·Cellular and Molecular Life Sciences : CMLS·Amnon Schlegel
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 23, 2012·World Journal of Gastroenterology : WJG·Neven BaršićMarko Duvnjak
Dec 25, 2012·Toxicologic Pathology·Banrida WahlangMatt C Cave
Jan 5, 2013·Toxicology·Xavier TekpliDominique Lagadic-Gossmann
Jan 9, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Yoshihisa OkadaYoshito Itoh
Feb 26, 2013·The Journal of Pharmacology and Experimental Therapeutics·Yu-Miao WeiPin-Lan Li
Jun 15, 2013·Oxidative Medicine and Cellular Longevity·Jie LiuCurtis D Klaassen
Jul 24, 2013·Journal of Gastroenterology and Hepatology·Yoon Seok Roh, Ekihiro Seki
Oct 9, 2013·Clinics in Liver Disease·Vaishali Patel, Arun J Sanyal
Mar 4, 2014·World Journal of Gastroenterology : WJG·Kenichiro YasutakeMunechika Enjoji
Jun 7, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Erdinc SezginKai Simons
Jul 18, 2014·Journal of Lipid Research·Lourdes Cruz-Garcia, Amnon Schlegel

❮ Previous
Next ❯

Citations

Feb 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saleh A AlmatroodiArshad Husain Rahmani
Feb 1, 2020·Animal Frontiers : the Review Magazine of Animal Agriculture·Tsegay TeameZhigang Zhou
Jun 22, 2021·Biochimie·Stéphane OrlowskiEric Bruckert

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
fluorescence microscopy

Software Mentioned

Fiji
GraphPad Prism5
ImageJ
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.