Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments

Journal of Nanobiotechnology
Chia-Wei LeeChau-Hwang Lee

Abstract

Cell membranes exhibit abundant types of responses to external stimulations. Intuitively, membrane topography should be sensitive to changes of physical or chemical factors in the microenvironment. We employed the non-interferometric wide-field optical profilometry (NIWOP) technique to quantify the membrane roughness of living neuroblastoma cells under various treatments that could change the mechanical properties of the cells. The membrane roughness was reduced as the neuroblastoma cell was treated with paclitaxel, which increases cellular stiffness by translocating microtubules toward the cell membranes. The treatment of positively charged gold nanoparticles (AuNPs) showed a similar effect. In contrast, the negatively charged AuNPs did not cause significant changes of the membrane roughness. We also checked the membrane roughness of fixed cells by using scanning electron microscopy (SEM) and confirmed that the membrane roughness could be regarded as a parameter reflecting cellular mechanical properties. Finally, we monitored the temporal variations of the membrane roughness under the treatment with a hypertonic solution (75 mM sucrose in the culture medium). The membrane roughness was increased within 1 h but returned to the ...Continue Reading

References

Nov 23, 2007·Optics Letters·Chau-Hwang LeeWan-Chen Lin
Feb 12, 2009·ACS Nano·Jason ReedJames K Gimzewski
Apr 7, 2010·Physical Review Letters·Chien-Hong ChenChau-Hwang Lee
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Valentin LulevichGang-yu Liu
Jun 21, 2011·Biochemistry·Keisuke IkedaKatsumi Matsuzaki
Feb 4, 2012·Journal of Cell Science·Omar F ZouaniMarie-Christine Durrieu
Aug 3, 2012·Micron : the International Research and Review Journal for Microscopy·Elena K KozlovaArtem N Kuzovlev
Jun 1, 2013·Advanced Materials·Min ZhangChen Wang
Feb 21, 2014·Molecular Neurobiology·Xiaoguang YangJames C-M Lee
Feb 26, 2015·Traffic·Anna BauereissTeja W Groemer

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Methods Mentioned

BETA
chemical treatments
scanning electron microscopy
environmental stress
AFM
confocal microscopy

Software Mentioned

NIWOP

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