PMID: 2493740Mar 1, 1989

Membrane site of action of CO2 on colonic sodium absorption

The American Journal of Physiology
A N Charney, R W Egnor

Abstract

Increases in ambient CO2 tension increase colonic sodium absorption by increasing mucosal to serosal sodium flux. We examined the membrane site of CO2 action by utilizing the polyene antibiotic nystatin to create aqueous pores in the apical membrane. Under these conditions, the basolateral rather than the apical membrane is rate limiting for sodium absorption. Pairs of stripped rat distal colonic segments were mounted in modified Ussing chambers in a Ringer-HCO3 solution gassed with either 3% CO2-97% O2 or 11% CO2-89% O2. Mucosal-to-serosal 22Na and 36Cl fluxes were measured under short-circuited conditions, and ouabain-sensitive absorption was calculated before and after the addition of mucosal nystatin 300 U/ml. Ouabain-sensitive sodium absorption was fivefold greater at 11% CO2 than at 3% CO2 before nystatin addition. Nystatin increased short-circuit current (Isc), transcolonic conductance (Gt) and ouabain-sensitive sodium absorption at 3% CO2 but only increased Isc and Gt at 11% CO2. The levels of sodium absorption at 3% and 11% CO2 after nystatin were equal and identical to the level measured at 11% CO2 in the absence of nystatin. Ouabain-sensitive chloride absorption was similar at 3% and 11% CO2 in the absence of nystati...Continue Reading

References

Mar 28, 1979·The Journal of Membrane Biology·N K WillsD C Eaton
Oct 1, 1977·The Journal of General Physiology·S A LewisJ M Diamond
Oct 1, 1977·The Journal of Membrane Biology·J M RussellM S Brodwick
Jan 1, 1986·The Journal of Membrane Biology·S Grinstein, A Rothstein
Jun 1, 1988·The Journal of Clinical Investigation·D S GoldfarbA N Charney
Oct 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·S F Phillips, P F Schmalz
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Aug 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·Robert BridgesB Simon

Citations

Oct 1, 1990·Diseases of the Colon and Rectum·W E Roediger
Jan 6, 2005·World Journal of Gastroenterology : WJG·Antti-J KiveläSeppo Parkkila
Aug 15, 2002·American Journal of Physiology. Cell Physiology·A N CharneyGurdip S Sidhu
Jan 1, 1994·JPEN. Journal of Parenteral and Enteral Nutrition·W E Roediger

Related Concepts

Carbon Dioxide
Chloride Ion Level
Taenia Coli
Electric Conductivity
Hydrogen-Ion Concentration
Intestinal Absorption
Structure of Intestinal Gland
Resting Potentials
Stamycin
Ouabain

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