Membrane supported liquid-liquid-liquid microextraction combined with field-amplified sample injection CE-UV for high-sensitivity analysis of six cardiovascular drugs in human urine sample

Electrophoresis
Xiaoqing ZhouBin Hu

Abstract

An effective dual preconcentration method involving off-line membrane supported liquid-liquid-liquid microextraction (MS-LLLME) and on-line field-amplified sample injection (FASI) was proposed for the extraction of six cardiovascular drugs, including mexiletine, xylocaine, propafenone, propranolol, metoprolol, and carvedilol from aqueous solution prior to CE-UV. In MS-LLLME, the analytes were extracted from 9 mL sample solution into toluene, and then back extracted into a drop of acceptor phase of 10 μL 20 mmol/L acetic acid. After that, the acceptor phase was directly introduced into CE for FASI without any modification. In FASI process, water plug was hydrodynamically injected (50 mbar, 3 s) into the capillary prior to sample injection (+6 kV, 18 s). Six target analytes were separated in less than 10 min at 25°C with a BGE consisting of 70 mmol/L Tris-H3 PO4 (pH 2.2) containing 10% v/v methanol. Under the optimized conditions, LODs obtained by the proposed MS-LLLME-FASI-CE-UV method were in the range of 0.02-0.82 μg/L (based on S/N = 3) with enrichment factors of 546- to 7300-fold for the target analytes. The RSDs of the developed method were in the range of 6.7-12.9% (n = 7). Good linearity (R(2) = 0.9928-0.9997) was obtaine...Continue Reading

References

Mar 30, 2005·Journal of Chromatography. a·Zhao-Xiang Zhang, You-Zhao He
Apr 1, 2006·Journal of Chromatography. a·Mohammad RezaeeSana Berijani
Jul 11, 2006·Journal of Chromatography. a·Chanbasha BasheerHian Kee Lee
Jun 11, 2008·Clinical Cardiology·Steven G ChrysantBilly Dimas
Jul 21, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pawel OlszowyBoguslaw Buszewski

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