Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2

Journal of Cell Science
Wassim Abou-KheirD Cox

Abstract

Wiskott-Aldrich syndrome protein (WASP)-family verprolin homologous (WAVE) proteins play a major role in Rac-induced actin dynamics, but Rac does not bind directly to WAVE proteins. It has been proposed that either the insulin receptor substrate protein 53 (IRSp53) or a complex of proteins containing Abelson interactor protein 1 (Abi1) mediates the interaction of WAVE2 and Rac. Depletion of endogenous IRSp53 by RNA-mediated interference (RNAi) in a RAW/LR5 macrophage cell line resulted in a significant reduction of Rac1Q61L-induced surface ruffles and colony-stimulating factor 1 (CSF-1)-induced actin polymerization, protrusion and cell migration. However, IRSp53 was not essential for Fcgamma-R-mediated phagocytosis, formation of podosomes or for formation of Cdc42V12-induced filopodia. IRSp53 was found to be present in an immunoprecipitable complex with WAVE2 and Abi1 in a Rac1-activation-dependent manner in RAW/LR5 cells in vivo. Importantly, reduction of endogenous IRSp53 or expression of IRSp53 lacking the WAVE2-binding site (IRSp53DeltaSH3) resulted in a significant reduction in the association of Rac1 with WAVE2 and Abi1, indicating that the association of Rac1 with WAVE2 and Abi1 is IRSp53 dependent. While it has been pro...Continue Reading

References

Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·J D SmithM Miyata
Jan 1, 1997·Methods in Molecular Biology·E R Stanley
Nov 14, 1997·The Journal of Experimental Medicine·D CoxS Greenberg
Jun 12, 1998·The Journal of Cell Biology·W E AllenG E Jones
Jun 24, 1998·The Journal of Clinical Investigation·T RajavashisthA Lusis
Jul 23, 1998·British Journal of Haematology·D ZichaA J Thrasher
Jun 25, 1999·Biochemical and Biophysical Research Communications·S SuetsuguT Takenawa
Mar 21, 2001·The Journal of Experimental Medicine·E Y LinJ W Pollard
Nov 7, 2001·Current Biology : CB·S KrugmannA Hall
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Gareth E JonesAdrian Thrasher
Jun 11, 2002·Biochemical and Biophysical Research Communications·Hiroaki Miki, Tadaomi Takenawa
Aug 16, 2002·Nature·Sharon EdenMarc W Kirschner
Jul 3, 2003·Trends in Cell Biology·Stefan Linder, Martin Aepfelbacher
Jul 25, 2003·Nature·Daisuke YamazakiTadaomi Takenawa
Sep 17, 2003·The Journal of Cell Biology·Stephen L RogersRonald D Vale
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Dec 31, 2003·Developmental Biology·Myrto Raftopoulou, Alan Hall
Feb 7, 2004·The EMBO Journal·Anika SteffenTheresia E B Stradal
Mar 30, 2004·Nature Cell Biology·Metello InnocentiGiorgio Scita
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alexis GautreauMarc W Kirschner
Jun 9, 2004·Trends in Cell Biology·Theresia E B StradalGiorgio Scita
Jul 29, 2004·Blood·Atsushi OdaTadaomi Takenawa
Nov 3, 2004·Trends in Cell Biology·Fiona J Pixley, E Richard Stanley
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akira YamauchiMary C Dinauer
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yan LengKatherine Siminovitch
Jan 28, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeonghoon ChoiEunjoon Kim
May 19, 2005·Molecular and Cellular Biology·Beth A ConnollyRachel J Buchsbaum
Jun 3, 2005·The Journal of Biological Chemistry·Jing ShiJames E Casanova
May 17, 2006·The Journal of Cell Biology·Shiro SuetsuguTadaomi Takenawa
Sep 28, 2006·The Journal of Biological Chemistry·Shiro SuetsuguShigeyuki Yokoyama

❮ Previous
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Citations

Feb 18, 2014·Progress in Neurobiology·Raphael Lamprecht
Jul 23, 2011·Nature Reviews. Cancer·Evanthia T RoussosAntonia Patsialou
Jul 10, 2008·Nature Reviews. Molecular Cell Biology·Joel A Swanson
Dec 20, 2011·The Journal of Biological Chemistry·Wah Ing GohSohail Ahmed
Aug 29, 2012·Molecular and Cellular Biology·Jing-Ling HoonCheng-Gee Koh
Jul 11, 2009·Genome Biology·Shusaku Kurisu, Tadaomi Takenawa
Aug 21, 2009·Journal of Cell Science·Corinne Albiges-RizoMarc R Block
May 15, 2010·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Tadaomi Takenawa
Apr 30, 2008·FEBS Letters·Markus Ladwein, Klemens Rottner
Feb 24, 2011·Cellular Signalling·Athanassios Dovas, Dianne Cox
Mar 2, 2011·Archives of Biochemistry and Biophysics·Haein ParkDianne Cox
Dec 8, 2009·Seminars in Cell & Developmental Biology·Shiro SuetsuguYosuke Senju
May 6, 2015·Small GTPases·Yingyu Mao, Silvia C Finnemann
Nov 17, 2009·Immunological Reviews·Jinyi ZhangKatherine A Siminovitch
Dec 5, 2008·Traffic·Silvia MassariGrazia Pietrini
Jan 22, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Emmanuel Derivery, Alexis Gautreau
Jun 27, 2014·Small GTPases·Pirjo SpuulElisabeth Génot
Feb 9, 2012·Journal of Oncology·Kazuhide Takahashi
May 28, 2013·Cellular Signalling·Sebastian Lewis-SaravalliAudrey Claing
Jun 21, 2012·Small GTPases·Bart-Jan de Kreuk, Peter L Hordijk
Apr 5, 2013·PloS One·Tsukasa OikawaShiro Suetsugu
Sep 16, 2015·PLoS Pathogens·Qian ShuCarolyn B Coyne
Aug 26, 2016·Immunological Reviews·Roni LevinJohnathan Canton
Jan 25, 2008·Trends in Cell Biology·Giorgio ScitaShiro Suetsugu
Nov 3, 2012·Seminars in Cell & Developmental Biology·Khalid Sossey-Alaoui
Jan 1, 2012·Membranes·Fatemeh Safari, Shiro Suetsugu
Mar 11, 2011·The Journal of Biological Chemistry·Haein Park, Dianne Cox
Sep 4, 2008·Journal of Leukocyte Biology·Hang DinhJohn A Hamilton
Mar 21, 2019·Molecular Biology of the Cell·David J Kast, Roberto Dominguez

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