Abstract
During the rolling of human neutrophils on the endothelium, tethers (cylindrical membrane tubes) are likely extracted from the neutrophil. Tether extraction reduces the force imposed on the adhesive bond between the neutrophil and endothelium, thereby facilitating the rolling. However, whether tethers can be extracted from the endothelium is still unknown. Here, with the micropipette-aspiration technique, we show that tethers can be extracted from either suspended or attached human umbilical vein endothelial cells. We also show that a linear relationship between the pulling force and tether growth velocity exists and this relationship does not depend on the receptor type (used to impose point forces), tumor necrosis factor-alpha stimulation, or cell attachment state. With linear regression, we determined that the threshold force was 50 pN and the effective viscosity was 0.50 pN.s/microm. Therefore, tethers might be simultaneously extracted from the neutrophil and endothelial cell during the rolling and, more importantly, the endothelial cell might contribute much more to the total composite tether length than the neutrophil. Compared with tether extraction from the neutrophil alone, simultaneous tether extraction results in a l...Continue Reading
References
Feb 1, 1991·The Journal of Cell Biology·K L MooreR P McEver
May 31, 1991·Cell·M B Lawrence, T A Springer
Aug 2, 1990·Nature·T A Springer
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeC R Minick
Feb 1, 1995·The Journal of Cell Biology·K L MooreR P McEver
May 1, 1995·Annals of Biomedical Engineering·R E Waugh, R G Bauserman
Mar 1, 1995·Biophysical Journal·J Dai, M P Sheetz
Jan 1, 1995·Annual Review of Physiology·T A Springer
Jan 18, 1996·Nature·E B FingerT A Springer
Jan 1, 1996·Biophysical Journal·F M HochmuthM P Sheetz
Mar 1, 1996·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·K E NormanK Ley
Nov 1, 1996·Biophysical Journal·J Y Shao, R M Hochmuth
Dec 1, 1996·Circulation Research·E R DamianoK Ley
Feb 10, 1997·The Journal of Cell Biology·M B LawrenceK Ley
Feb 4, 1998·Biophysical Journal·J W PiperC Zhu
May 30, 1998·Gastroenterology·J Panés, D N Granger
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Y ShaoR M Hochmuth
Jul 2, 1999·Biophysical Journal·J Y Shao, R M Hochmuth
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E J KunkelK Ley
May 3, 2000·The Journal of Cell Biology·D W Schmidtke, S L Diamond
Feb 28, 2002·Biophysical Journal·Zhiwei LiWilliam E Brownell
Mar 28, 2002·Biophysical Journal·Eric Y H ParkMichael B Lawrence
May 23, 2002·Biophysical Journal·Robert M Hochmuth, Warren D Marcus
Aug 15, 2002·The Journal of Cell Biology·Tadayuki YagoRodger P McEver
Aug 22, 2002·Journal of Biomechanical Engineering·Jin-Yu Shao, Jinbin Xu
Jan 24, 2003·Annals of Biomedical Engineering·Warren D Marcus, Robert M Hochmuth
May 9, 2003·Nature·Bryan T MarshallCheng Zhu
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Jin-Yu ShaoPeng Guo
Citations
Jul 9, 2010·Annals of Biomedical Engineering·Yong ChenJin-Yu Shao
May 4, 2010·Molecular Neurobiology·Xiaoguang YangJames C-M Lee
Aug 29, 2006·PLoS Biology·Manu ForeroViola Vogel
May 6, 2009·Journal of Molecular Recognition : JMR·Charles M CuerrierMichel Grandbois
Jun 24, 2010·Biochemical and Biophysical Research Communications·Johanne L PythonWilliam H Guilford
Mar 10, 2016·Biophysical Journal·Yong ChenJin-Yu Shao
Oct 31, 2006·Biophysical Journal·Yan Yu, Jin-Yu Shao
Jun 26, 2007·Biophysical Journal·Baoyu LiuJin-Yu Shao
Nov 14, 2006·Biophysical Journal·Gaurav GirdharJin-Yu Shao
Jun 16, 2015·Frontiers in Neuroscience·Donghui ZhuSholpan Askarova
Aug 21, 2007·Biophysical Journal·Gaurav Girdhar, Jin-Yu Shao
Apr 21, 2011·Biophysical Journal·Serge MostowyFrank Lafont
Jan 4, 2013·Biophysical Journal·Baoyu Liu, Jin-Yu Shao
Sep 27, 2005·Biophysical Journal·Mingzhai SunMichel Grandbois
Aug 16, 2005·Biophysical Journal·K E EdmondsonS L Diamond
Aug 30, 2016·Cell Reports·Rhodaba EbadyTara J Moriarty
Jul 26, 2018·Nanoscale·Berta Gumí-AudenisMarina I Giannotti
Aug 1, 2007·Physical Biology·Basarab G HosuGabor Forgacs
Nov 13, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Kristopher R SchumacherAlexander A Spector
Feb 22, 2007·Blood·Shaun P Jackson
Jan 1, 2009·Cellular and Molecular Bioengineering·Yong ChenJin-Yu Shao
Nov 3, 2006·American Journal of Physiology. Cell Physiology·Yong ChenJin-Yu Shao
Jun 7, 2007·Journal of Cell Science·Mingzhai SunGabor Forgacs
May 15, 2021·Optics Express·Xuanling LiMing Li
Jun 3, 2021·Life·Andrea CugnoKlaus Ley
May 6, 2006·Ultramicroscopy·Pierre-Henri PuechDaniel J Muller