Membrane-tethered syntaxin-4 locally abrogates E-cadherin function and activates Smad signals, contributing to asymmetric mammary epithelial morphogenesis

Journal of Cellular Biochemistry
Yuina HiroseYohei Hirai

Abstract

Spatial and temporal epithelial-mesenchymal transition (EMT) is a critical event for the generation of asymmetric epithelial architectures. We found that only restricted cell populations in the morphogenic mammary epithelia extrude syntaxin-4, a plasmalemmal t-SNARE protein, and that epithelial cell clusters with artificial heterogenic presentation of extracellular syntaxin-4 undergo asymmetric morphogenesis. A previous study revealed that inducible expression of cell surface syntaxin-4 causes EMT-like cell behaviors in the clonal mammary epithelial cells, where laminin-mediated signals were abolished so that cells readily succumb to initiate EMT. The present study added new mechanistic insight into syntaxin-4-driven EMT-like cell behaviors. Extracellular syntaxin-4 directly perturbs E-cadherin-mediated epithelial cell-cell adhesion and activates Smad signals. We found that the epithelial cells activated Smad2/3 upon induction of expression of extracellular syntaxin-4, leading to the upregulation of certain transcriptional targets of these TGF-β signaling mediators. Intriguingly, however, mRNA expression of canonical EMT initiators, such as Snail and Slug, was unchanged. In addition, E-cadherin protein was steeply decreased, ye...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C SchmidhauserG F Casperson
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K G DanielsonD Medina
Feb 14, 1998·The Journal of Cell Biology·Y HiraiM J Bissell
Mar 11, 2000·Molecular Cell·C DongP J Goldschmidt-Clermont
Dec 12, 2001·Genome Biology·F Y TengB L Tang
Jul 31, 2003·Trends in Cell Biology·Derek C RadiskyMina J Bissell
Mar 30, 2004·Trends in Endocrinology and Metabolism : TEM·Christopher D JohnJulia C Buckingham
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jean Paul Thiery, Jonathan P Sleeman
Jun 6, 2007·Traffic·Clare E Futter, Ian J White
Oct 11, 2008·Current Opinion in Cell Biology·Alexander Aulehla, Olivier Pourquié
Nov 8, 2008·BJU International·Jessica A MandevilleIan C Summerhayes
Feb 5, 2009·Nature Reviews. Molecular Cell Biology·Derek C RadiskyMina J Bissell
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marjo de GraauwBob van de Water
Sep 8, 2010·EMBO Molecular Medicine·Sabine MaschlerHartmut Beug
Jan 10, 2012·Biochemical and Biophysical Research Communications·Nanako KadonoYohei Hirai
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jul 23, 2013·Cancer Research·Jamie L BascomMina J Bissell
Oct 31, 2013·Current Cancer Drug Targets·Yifan WangBinhua P Zhou
Dec 9, 2014·Cell Adhesion & Migration·Sarah Phillips, Charlotte Kuperwasser
Oct 7, 2015·Molecular Cancer·André Filipe Vieira, Joana Paredes
May 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tong GaoLian-Hong Li
Jan 31, 2017·Cancer Letters·Ross L PearlmanFarrukh Afaq
Jun 19, 2017·European Journal of Cell Biology·Huimei RenEberhard Hildt
Aug 30, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qing ChenMing-Qing Gao

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