Membrane topology of outer membrane protein AlgE, which is required for alginate production in Pseudomonas aeruginosa.

Applied and Environmental Microbiology
Iain D HayBernd H A Rehm

Abstract

The ubiquitous opportunistic human pathogen Pseudomonas aeruginosa secretes a viscous extracellular polysaccharide, called alginate, as a virulence factor during chronic infection of patients with cystic fibrosis. In the present study, it was demonstrated that the outer membrane protein AlgE is required for the production of alginate in P. aeruginosa. An isogenic marker-free algE deletion mutant was constructed. This strain was incapable of producing alginate but did secrete alginate degradation products, indicating that polymerization occurs but that the alginate chain is subsequently degraded during transit through the periplasm. Alginate production was restored by introducing the algE gene. The membrane topology of the outer membrane protein AlgE was assessed by site-specific insertions of FLAG epitopes into predicted extracellular loop regions.

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Citations

Jul 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·John C WhitneyP Lynne Howell
Mar 19, 2013·Applied and Environmental Microbiology·Zahid U RehmanBernd H A Rehm
Feb 26, 2013·Applied and Environmental Microbiology·Aamir GhafoorBernd H A Rehm
Jan 22, 2013·Applied and Environmental Microbiology·Zahid U Rehman, Bernd H A Rehm
Sep 17, 2013·Microbial Biotechnology·Iain D HayBernd H A Rehm
Apr 30, 2015·Applied Microbiology and Biotechnology·Yajie WangBernd H A Rehm
Jun 16, 2015·Frontiers in Microbiology·Jochen SchmidBernd Rehm
Nov 9, 2016·Scientific Reports·Yajie WangBernd H A Rehm
Mar 5, 2017·Applied and Environmental Microbiology·M Fata MoradaliBernd H A Rehm
Dec 15, 2015·Applied and Environmental Microbiology·Susan MalekiHelga Ertesvåg
Jan 5, 2020·Applied Microbiology and Biotechnology·Wankuson ChanasitBernd H A Rehm
Jan 17, 2014·Environmental Microbiology·Iain D HayBernd H A Rehm
May 13, 2017·Frontiers in Immunology·André Alex GrassmannAlan John Alexander McBride
Jan 26, 2021·International Journal of Biological Macromolecules·Xiaqing Sun, Jianfa Zhang

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