Membrane topology of the Salmonella enterica serovar Typhimurium Group B O-antigen translocase Wzx

FEMS Microbiology Letters
Monica M Cunneen, Peter R Reeves

Abstract

The O-antigen translocase, Wzx, is involved in translocation of bacterial polysaccharide repeat units across the cytoplasmic membrane, and is an unusually diverse, highly hydrophobic protein, with high numbers of predicted alpha-helical transmembrane segments (TMS). The Salmonella enterica serovar Typhimurium Group B O-antigen Wzx was an ideal candidate for topological study as the O-antigen gene cluster is one of only a few that have been well characterized. The topology profile prediction for this protein was determined using five programs, with different recognition parameters, which consistently predict that 12 TMS are present. A membrane topology model was constructed by analysis of lacZ and phoA gene fusions at randomly selected and targeted fusion sites within wzx. Enzyme activity of these, and full-length C-terminal fusion proteins, confirmed the 12-TMS topology for this Wzx, and also indicated that the C-terminus was located within the cytoplasm, which is consistent with the predicted topology.

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Citations

Aug 20, 2009·ACS Chemical Biology·Sumana Sanyal, Anant K Menon
Aug 4, 2009·Journal of Bacteriology·Pradeep VasudevanDavid L Popham
Nov 2, 2014·Canadian Journal of Microbiology·Salim T Islam, Joseph S Lam
Sep 29, 2012·Environmental Microbiology·Salim T Islam, Joseph S Lam

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