Membrane trafficking factors are involved in the hypotonic activation of the taurine channel in the little skate (Raja erinacea) red blood cell

Journal of Experimental Zoology. Part A, Comparative Experimental Biology
Amanda B PufferLeon Goldstein

Abstract

In response to volume expansion, red blood cells of the little skate (Raja erinacea) initially swell and then release small organic compounds and osmotically obligated water in what is called a regulatory volume decrease (RVD) to restore cell volume. One of the major intracellular solutes lost during this process is the non-metabolized beta amino acid taurine. This hypoosmotic-induced increase in cell taurine permeability requires the anion exchanger, skAE1. The abundance of this transporter increases on the surface plasma membrane by a process of exocytosis. The second-messenger pathways involved in exocytosis of skAE1 were investigated with the use of inhibitors which affect membrane trafficking. Hypoosmotic-stimulated taurine uptake was significantly decreased by 42% with wortmannin, a phosphatidylinositol 3-kinase (PI3 kinase) inhibitor. Additional evidence for the involvement of PI3K was obtained with a second inhibitor, LY294002, which decreased the hypoosmotic-stimulated taurine uptake by 28%. The state of actin is also involved, as the actin filament depolymerizer latrunculin B decreased hypoosmotic-stimulated taurine uptake by approximately 40%. Although hypoosmotic conditions did not stimulate changes in the distribut...Continue Reading

References

Oct 19, 1999·Microscopy Research and Technique·T TsakiridisG P Downey
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·S QinP B Chock
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Gómez-MóutonC Martínez-A
Aug 21, 2001·Pflügers Archiv : European journal of physiology·R FrancoH Pasantes-Morales
Sep 13, 2002·The Biochemical Journal·Patricia NecoLuis M Gutiérrez
Oct 12, 2002·Journal of Cell Science·Dorothy I MundyGeorge S Bloom
Aug 13, 2003·Kidney International·Damien du CheyronJosiane Poggioli
Mar 3, 2004·Neurochemical Research·Deborah F Perlman, Leon Goldstein
Apr 15, 2004·Endocrine Reviews·Robert T WatsonJeffrey E Pessin
Jun 1, 2004·Molecular and Cellular Biology·Avirup BoseMichael P Czech
Jul 20, 2004·The Journal of Biological Chemistry·Mark W MuschLeon Goldstein
Apr 30, 2005·Journal of Applied Physiology·Jill L SmithJonathan S Fisher

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Citations

Dec 15, 2010·The Journal of Membrane Biology·Alina B ElperinDouglas B Light
Feb 22, 2011·Fish Physiology and Biochemistry·Chloe WormserDouglas B Light
Oct 24, 2008·Acta Physiologica·I H LambertS F Pedersen
Feb 4, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Osvaldo CharaPablo J Schwarzbaum
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen

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