Membrane type-1 matrix metalloprotease and stromelysin-3 cleave more efficiently synthetic substrates containing unusual amino acids in their P1' positions.

The Journal of Biological Chemistry
A MuchaV Dive

Abstract

The influence of the substrate P1' position on the specificity of two zinc matrix metalloproteases, membrane type-1 matrix metalloprotease (MT1-MMP) and stromelysin-3 (ST3), was evaluated by synthesizing a series of fluorogenic substrates of general formula dansyl-Pro-Leu-Ala-Xaa-Trp-Ala-Arg-NH2, where Xaa in the P1' position represents unusual amino acids containing either long arylalkyl or alkyl side chains. Our data demonstrate that both MT1-MMP and ST3 cleave substrates containing in their P1' position unusual amino acids with extremely long side chains more efficiently than the corresponding substrates with natural phenylalanine or leucine amino acids. In this series of substrates, the replacement of leucine by S-para-methoxybenzyl cysteine increased the kcat/Km ratio by a factor of 37 for MT1-MMP and 9 for ST3. The substrate with a S-para-methoxybenzyl cysteine residue in the P1' position displayed a kcat/Km value of 1.59 10(6) M-1 s-1 and 1.67 10(4) M-1 s-1, when assayed with MT1-MMP and ST3, respectively. This substrate is thus one of the most rapidly hydrolyzed substrates so far reported for matrixins, and is the first synthetic peptide efficiently cleaved by ST3. These unexpected results for these two matrixins sugges...Continue Reading

References

May 15, 1991·Analytical Biochemistry·S Netzel-ArnettH E Van Wart
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Feb 1, 1994·Nature Structural Biology·T StamsB Rubin
Sep 1, 1995·The Journal of Biological Chemistry·P AnglardP Basset
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A OkadaP Basset
May 23, 1995·Biochemistry·M F BrownerA L Castelhano
Jun 1, 1994·Proteins·J C SpurlinoT A Pulvino
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
Oct 1, 1995·Protein Science : a Publication of the Protein Society·J W BeckerJ D Hermes
Nov 1, 1995·Chemical & Pharmaceutical Bulletin·K TamakiY Sugimura
Jun 1, 1996·Seminars in Cancer Biology·W G Stetler-StevensonM Corcoran
Feb 1, 1996·Journal of Biochemistry·H Sato, M Seiki
Nov 1, 1996·Chemistry & Biology·L M Coussens, Z Werb
Mar 14, 1997·Journal of Medicinal Chemistry·C K EsserW K Hagmann
May 1, 1997·Critical Reviews in Oncology/hematology·P BassetM C Rio

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Nov 1, 2001·Respiratory Research·W C Parks, S D Shapiro
Jun 1, 2014·Biochemical and Biophysical Research Communications·Amanda HaageIan C Schneider
Aug 31, 2012·Chembiochem : a European Journal of Chemical Biology·Anna Knapinska, Gregg B Fields
May 20, 2006·Medicinal Research Reviews·Magdalini MatziariAthanasios Yiotakis
Oct 9, 2019·Chemistry, an Asian Journal·Izabela MaluchMarcin Drag
May 3, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda Haage, Ian C Schneider
May 10, 2003·The Journal of Biological Chemistry·Weijun PanCharles F Albright
Apr 23, 2008·Current Protocols in Protein Science·Linda Troeberg, Hideaki Nagase
Feb 17, 2006·The Journal of Biological Chemistry·Laurent DevelVincent Dive
Feb 6, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kyle J IsaacsonHamidreza Ghandehari
May 5, 2009·Biomacromolecules·Alex A AimettiKristi S Anseth
Dec 26, 2001·Chemical Reviews·M WhittakerA J Gearing
Jan 9, 2004·Journal of Medicinal Chemistry·Magdalini MatziariAthanasios Yiotakis
Feb 6, 2007·Bioorganic & Medicinal Chemistry·Rajeshwar P Verma, Corwin Hansch

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