PMID: 5257143Jun 1, 1969Paper

Membranes of animal cells. 3. Amino acid incorporation by isolated surface membranes

Proceedings of the National Academy of Sciences of the United States of America
M C Glick, L Warren

Abstract

Surface membranes isolated from mouse fibroblasts contain RNA organized in ribosome-like particles. These surface membranes can incorporate radioactive amino acids into material insoluble in hot trichloroacetic acid. Characterization of the radioactive products indicated that they are large polypeptides. Most requirements and inhibitors of the system were similar to those of the microsomal system of the endoplasmic reticulum. Further comparisons were made with microsomal and pH 5 fractions prepared from L-cells by standard procedures and by two methods attempting to simulate the conditions necessary for isolating surface membranes. The amount of L-leucine incorporated by the surface membranes per mg of RNA was comparable to the values obtained by the microsomal and pH 5 fractions prepared by standard procedures. In contrast, the activity of the two other microsomal fractions was less than 10 per cent of this amount. The most striking difference noted was in the products formed by the surface membranes and by the microsomal systems. These products were demonstrated to be quite different by electrophoresis on acrylamide gels. It is suggested that the surface membranes participate actively in protein synthesis in vivo, and that, b...Continue Reading

References

Jan 1, 1968·Zeitschrift für Zellforschung und mikroskopische Anatomie·J K Koehler
Sep 28, 1967·Journal of Molecular Biology·E Knight, J E Darnell
Dec 1, 1961·The Analyst·W C HUTCHISON, H N MUNRO

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Citations

Sep 1, 1971·The Journal of Membrane Biology·D M Brunette, J E Till
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R A LernerD A Goldstein
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Rieber, J Bacalao
Oct 1, 1971·The Journal of Cell Biology·A G UlsamerE D Korn
Apr 1, 1971·The Journal of Experimental Medicine·R L NachmanJ G Hirsch
Dec 1, 1970·Biochimica Et Biophysica Acta·M C GlickL Warren
Apr 24, 1970·Biochemical and Biophysical Research Communications·B R Fridlender, F O Wettstein
Oct 1, 1974·Journal of Theoretical Biology·E Mayhew
Jun 1, 1971·Experimental Cell Research·K M AndersonM Slavik
Dec 5, 1974·Journal of Molecular Biology·M Noll, M M Burger
Dec 1, 1972·Archives of Oral Biology·M S Nijjar, E T Pritchard
Oct 1, 1970·Journal of Neurochemistry·M BurnelW J Moore
Dec 22, 1971·Nature: New Biology·M R HallR A Lerner
Sep 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·A González-RoblesA Martínez-Palomo
Nov 1, 1972·Infection and Immunity·N Hahon, H L Eckert
Mar 1, 1974·Bacteriological Reviews·J W CostertonK J Cheng
Apr 1, 1977·Journal of Bacteriology·T J MacAlisterJ W Costerton

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