PMID: 639824Apr 1, 1978Paper

Membranes rich in acetylcholine receptor: characterization and reconstitution to excitable membranes from exogenous lipids

European Journal of Biochemistry
W Schiebler, Ferdinand Hucho

Abstract

Characterization of acetylcholine-receptor-enriched membranes from Torpedo californica electric tissue by negative-staining electron-microscopy and by lipid analysis is described. The protein/lipid ratio is 70%/30%. The lipids consist of 70% phospholipids (46% phosphatidylcholine, 31% phosphatidylethanolamine, 14% phosphatidylserine, 7% sphingomyelin, 2% phosphatidylinositol of the phospholipids determined) and 20% cholesterol. The acetylcholinesterase-enriched membranes show a similar composition. The only differences are a lower protein/lipid ratio (45%/55%) and a lower phosphatidylcholine/sphingomyelin ratio of 39%/14% as compared to 46%/7% for the receptor-enriched membranes. A method of preparing single-walled phosphatidylcholine vesicles by gel filtration on Sephadex G50 according to Brunner et al. (Biochem. Biophys. Acta, 455, 322--331, 1976) is used to recombine the lipid-depleted receptor complex with artificial lipid vesicles. Starting from a lipid mixture of 46% phosphatidylcholine, 31% phosphatidylethanolamine, 14% phosphatidylserine, 7% sphingomyelin, 2% phosphatidylinositol and 15% cholesterol we obtained vesicles associated with the acetylcholine receptor complex. These receptor vesicles are chemically excitable ...Continue Reading

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Citations

Dec 29, 1977·Molecular and Cellular Biochemistry·F Hucho, W Schiebler
Apr 1, 1988·Journal of Protein Chemistry·W Oberthür, F Hucho
Nov 1, 1983·Archives of Toxicology·D Kuhnen-ClausenF Hucho
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J H Nuske
Oct 1, 1982·Neuroscience·M G McNamee, E L Ochoa
Jan 1, 1989·Electron Microscopy Reviews·W KunathF Hucho
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H P ZingsheimJ Frank
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Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·A WattyF Hucho
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Oct 14, 1980·Biochemistry·J LindstromM Ellisman
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