Membranous and structural damage that occur during cryopreservation of human sperm may be time-related events

Fertility and Sterility
Pascal DesrosiersRobert Sullivan

Abstract

To evaluate sperm cryopreservation-induced injuries using sperm plasma membrane protein P34H and alpha-tubulin as two different subcellular compartment markers. Prospective experimental study. Academic hospital research center and fertility clinic. Semen samples obtained from healthy donors attending the fertility clinic. Sperm samples were either directly processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot experiments (control group) or cryopreserved in liquid nitrogen for different periods of time before being analyzed. SDS-PAGE, Western blotting, and densitometric quantification of P34H and alpha-tubulin before and after cryopreservation. Changes in protein quantification between the different groups as a result of sperm cryopreservation. In the 31 sperm samples processed for P34H evaluation, a 50% decrease is observed after sperm cryopreservation as compared to the control group. The alpha-tubulin immunoblotting of 41 sperm samples revealed a 200% increase in the protein detection in the group of cryopreserved sperm as compared to the control group. Contrary to the P34H detection, this change in alpha-tubulin immunodetected levels appears to be related to the cryopreservation ...Continue Reading

Citations

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