Abstract
Cognitive changes have been known in encephalitis but in Japanese encephalitis (JE) such studies are limited. This study aims at evaluating the spatial memory and learning and correlate with markers of cholinergic activity in the brain.12day old Wistar rats were inoculated with dose of 3×10(6)pfu/ml of JE virus. On 10, 33 and 48days post-inoculation (dpi), spatial memory and learning was assessed by Y maze. Brain biopsies from frontal cortex, corpus striatum, hippocampus and cerebellum were taken. Muscarinic cholinergic receptor was assayed by Quinuclidinyl benzylate (H3-QNB) binding, CHRM2 gene expression by real time PCR and choline acetyl transferase (ChAT) by Western blot. Spatial learning and memory showed significant decline in rats inoculated with JEV on 10 and 33dpi (47.5%, p<0.01; 30.2%, p<0.01). It started recovering on 48dpi. Muscarinic cholinergic receptors showed significant decrease in frontal cortex (31%, p=0.001; 26%, p=0.003), hippocampus (57%, p=0.001; 39.9%, p=0.002) and cerebellum (31.2%, p=0.008; 21.6%, p=0.007) but not in corpus striatum as compared to control. The mRNA expression of CHRM2 receptor gene showed significant decrease in the expression in frontal cortex (48%, p<0.001; 38%, p<0.01), hippocampus...Continue Reading
References
Dec 1, 1990·Journal of Neuroscience Research·E Giacobini
Dec 1, 1989·Biological Psychiatry·W DittrichK Schneider
Sep 25, 1986·Neuroscience Letters·A NordbergB Winblad
Dec 3, 1986·Neuroscience Letters·A Nordberg, B Winblad
May 31, 1985·Science·D C MashL T Potter
Aug 1, 1983·The Journal of Infectious Diseases·O NarayanR Rott
Jan 1, 1995·Acta Neuropathologica·A DesaiM Gourie-Devi
Jan 1, 1995·Neuroscience·E K PerryR H Perry
Dec 1, 1994·Journal of Neurology, Neurosurgery, and Psychiatry·U K MisraA Mathur
Sep 1, 1994·Journal of Applied Toxicology : JAT·V K KhannaP K Seth
Jan 1, 1994·Neuroepidemiology·R KumarJ Arockiasamy
Jan 1, 1995·Virus Genes·Y Becker
Apr 1, 1996·Journal of the Neurological Sciences·H DumrongpholP Govitrapong
Jul 1, 1993·Reviews in the Neurosciences·A H MohammedK Kristensson
Dec 1, 2001·Neuroreport·U GiesT Harkany
Dec 1, 2001·European Journal of Pharmacology·G J CareyV L Coffin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 14, 2003·Journal of Virology·Tom SolomonAlan D T Barrett
Jun 25, 2005·Current Alzheimer Research·Zhen Yan, Jian Feng
Oct 19, 2006·Neuroscience·M JamalI Ijiri
Mar 10, 2009·Brain Research·Sandeep KumarUsha Kant Misra
Jul 28, 2009·Brain Research·Usha Kant MisraGautam Palit
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andreea C BostanPeter L Strick
Aug 12, 2010·Neurotoxicology and Teratology·Verónica Mireya RodríguezMagda Giordano
May 11, 2011·Antiviral Research·Cynthia A SmeraskiJohn D Morrey
Nov 29, 2011·CNS Neuroscience & Therapeutics·Brian BalinJohn Schrogie
Apr 14, 2012·Neurotoxicology·Yun Chen
Sep 25, 2011·Journal of Bioterrorism & Biodefense·Monica A McArthur, Michael R Holbrook
Citations
Mar 31, 2016·Biological Trace Element Research·Kaveh TabrizianMohammad Sharifzadeh
Jul 6, 2016·Frontiers in Microbiology·Shannon E RoncaSlobodan Paessler
Aug 26, 2016·Reviews in the Neurosciences·Changzheng ZhangTifei Yuan
Jan 20, 2019·Neurochemical Research·Gal Hacohen-KleimanIllana Gozes
Aug 12, 2020·Journal of Neuroinflammation·Marion CléSara Salinas
Jan 7, 2021·Antioxidants·Prapimpun WongchitratRussel J Reiter
Feb 4, 2019·Experimental Neurology·Ihuoma NjokuCorina O Bondi