PMID: 9447250Feb 3, 1998Paper

Menadione-induced cytotoxicity to rat osteoblasts

Cellular and Molecular Life Sciences : CMLS
J S SunF J Lu

Abstract

Oxygen-derived free radical injury has been associated with several cytopathic conditions. Oxygen radicals produced by chondrocytes is an important mechanism by which chondrocytes induce matrix degradation. In the present study, we extend these observations by studying oxidative processes against osteoblasts. Osteoblasts were mixed in in vitro culture with 200 microM menadione. The cytotoxic effect of menadione-induced oxidative stress was monitored by lucigenin- or luminol-amplified chemiluminescence, tetrazolium assay and immunocytochemical study. Results showed that adding menadione induces an oxidative stress on osteoblasts, via superoxide and hydrogen peroxide production, that can be eradicated by superoxide dismutase (SOD) and catalase in a dose-dependent manner. Catalase and the appropriate concentration of dimethyl sulfoxide have a protective effect on cytotoxicity induced by menadione, whereas SOD does not. Menadione-treated osteoblasts have a strong affinity for annexin V, and the nuclei are strongly stained by TUNEL (TdT-mediated dUTP nick-end labelling). The results suggest that menadione-triggered production of reactive oxygen species leads to apoptosis of osteoblasts.

Citations

Aug 18, 2009·Apoptosis : an International Journal on Programmed Cell Death·Huan Ling LiangVani Nilakantan
Jun 25, 1999·Free Radical Biology & Medicine·J S SunY H Tsuang
Jun 4, 2008·Biological & Pharmaceutical Bulletin·Sumio MatznoKenji Matsuyama
Sep 11, 2008·The Journal of Oral Implantology·Wihaskoro SosrosenoFikri Ibrahim
Mar 6, 2012·Environmental Toxicology and Pharmacology·Jing GuoZengtian Sun
May 10, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jyh-Ding WeiRuei-Ming Chen
Sep 25, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·R CaricchioP L Cohen
Dec 1, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·I Laux, A Nel
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Feb 24, 2001·Plastic and Reconstructive Surgery·G M DryM L Cunningham
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