Menaquinone as well as ubiquinone as a bound quinone crucial for catalytic activity and intramolecular electron transfer in Escherichia coli membrane-bound glucose dehydrogenase.

The Journal of Biological Chemistry
Golam MustafaMamoru Yamada

Abstract

Escherichia coli membrane-bound glucose dehydrogenase (mGDH), which is one of quinoproteins containing pyrroloquinoline quinone (PQQ) as a coenzyme, is a good model for elucidating the function of bound quinone inside primary dehydrogenases in respiratory chains. Enzymatic analysis of purified mGDH from cells defective in synthesis of ubiquinone (UQ) and/or menaquinone (MQ) revealed that Q-free mGDH has very low levels of activity of glucose dehydrogenase and UQ2 reductase compared with those of UQ-bearing mGDH, and both activities were significantly increased by reconstitution with UQ1. On the other hand, MQ-bearing mGDH retains both catalytic abilities at the same levels as those of UQ-bearing mGDH. A radiolytically generated hydrated electron reacted with the bound MQ to form a semiquinone anion radical with an absorption maximum at 400 nm. Subsequently, decay of the absorbance at 400 nm was accompanied by an increase in the absorbance at 380 nm with a first order rate constant of 5.7 x 10(3) s(-1). This indicated that an intramolecular electron transfer from the bound MQ to the PQQ occurred. EPR analysis revealed that characteristics of the semiquinone radical of bound MQ are similar to those of the semiquinone radical of b...Continue Reading

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Citations

Apr 15, 2010·Journal of the American Chemical Society·Rodrigo Arias-CartinStéphane Grimaldi
Oct 4, 2013·BMC Systems Biology·Edwin H WintermutePamela A Silver
Nov 3, 2010·FEBS Letters·Renata TonhosoloAlejandro M Katzin
Jul 6, 2010·Biochimica Et Biophysica Acta·Beatrycze Nowicka, Jerzy Kruk
Jul 14, 2016·FEMS Microbiology Letters·István NagyWolfgang Baumeister
Jul 10, 2013·The Journal of Biological Chemistry·Prashant K SinghTina M Iverson
Jun 3, 2017·Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials·Kazuto IkemotoKazuo Mukai
Feb 12, 2019·Chemical Reviews·Kazuo Kobayashi

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