Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals.

Acta Neuropathologica Communications
Sarah FindaklyDavid R Raleigh

Abstract

Meningiomas are the most common primary intracranial tumors, but treatment options for meningioma patients are limited due to incomplete understanding of tumor biology. A small percentage of meningiomas harbor somatic variants in the Hedgehog pathway, a conserved gene expression program that is essential for development and adult stem cell homeostasis. Hedgehog signals are transduced through primary cilia, and misactivation of the Hedgehog pathway is known to underlie cancer. Nevertheless, the mechanisms of Hedgehog signaling in meningioma are unknown. Here, we investigate mechanisms of ciliary Hedgehog signaling in meningioma using tissue microarrays containing 154 human meningioma samples, NanoString transcriptional profiling, primary meningioma cells, pharmacology, and CRISPR interference. Our results reveal that meningiomas of all grades can express primary cilia, but that cilia are less prevalent among anaplastic tumors. Moreover, we find that expression of Smoothened alleles that are oncogenic in other contexts fail to activate the Hedgehog transcriptional program or promote proliferation in primary meningioma cells. These data reveal that meningiomas can express the subcellular structure necessary for canonical Hedgehog ...Continue Reading

References

Jun 21, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sylvia PüttmannWerner Paulus
Apr 14, 2010·Molecular Medicine·Ingrid LaurendeauIrene Szijan
Apr 20, 2011·Nature Reviews. Genetics·Philip W InghamClaudia Seger
Jan 22, 2013·Nature Genetics·Priscilla K BrastianosRameen Beroukhim
May 27, 2014·Nature Genetics·Robert T SweeneyRobert B West
Apr 25, 2018·Current Biology : CB·Galo GarciaJeremy F Reiter
Jan 17, 2019·Neuro-oncology·Priscilla K BrastianosUNKNOWN International Consortium on Meningiomas
Feb 2, 2019·The Journal of Clinical Investigation·David R Raleigh, Jeremy F Reiter
May 31, 2019·The American Journal of Dermatopathology·Ursula E LangJinah Kim
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Akash J PatelTiemo J Klisch
Oct 28, 2019·Journal of Neurosurgery·Mark W YoungbloodMurat Günel

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
fluorescence activated cell sorting
confocal microscopy

Clinical Trials Mentioned

NCT02523014

Software Mentioned

ImageJ
NanoString

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Cell Science
Markku Varjosalo, Jussi Taipale
Cold Spring Harbor Perspectives in Biology
Philip W Ingham
Tijdschrift Voor Diergeneeskunde
T Deden
The Veterinary Record
M J Maggs
© 2021 Meta ULC. All rights reserved