Mepe is expressed during skeletal development and regeneration

Histochemistry and Cell Biology
Chuanyong LuCéline Colnot

Abstract

a bone metabolism regulator that is expressed by osteocytes in normal adult bone. Here, we used an immunohistochemical approach to study whether Mepe has a role in murine long bone development and regeneration. Our data showed that Mepe protein was produced by osteoblasts and osteocytes during skeletogenesis, as early as 2 days postnatal. During the healing of non-stabilized tibial fractures, which occurs through endochondral ossification, Mepe expression was first detected in fibroblast-like cells within the callus by 6 days postfracture. By 10 and 14 days postfracture (the hard callus phase of repair), Mepe was expressed within late hypertrophic chondrocytes and osteocytes in the regenerating tissues. Mepe became externalized in osteocyte lacunae during this period. By 28 days postfracture (the remodeling phase of repair), Mepe continued to be robustly expressed in osteocytes of the regenerating bone. We compared the Mepe expression profile with that of alkaline phosphatase, a marker of bone mineralization. We found that both Mepe and alkaline phosphatase increased during the hard callus phase of repair. In the remodeling phase of repair, Mepe expression levels remained high while alkaline phosphatase activity decreased. We a...Continue Reading

References

Jun 1, 1986·Endocrinology·C S TamH Rasmussen
Mar 14, 1998·Clinical Endocrinology·A E NelsonR S Mason
May 3, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A X LeJ A Helms
Sep 11, 2002·Biochemical and Biophysical Research Communications·Rong GuoL Darryl Quarles
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zachary ThompsonJill A Helms
Jun 7, 2003·American Journal of Physiology. Endocrinology and Metabolism·L Darryl Quarles
Jul 23, 2003·Development·Céline ColnotJill A Helms

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Citations

Dec 15, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J M ZmudaUNKNOWN Osteoporotic Fractures in Men (MrOS) Study Group
Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Nov 12, 2005·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Jul 9, 2011·Journal of Bone and Mineral Metabolism·Yasuo ImanishiMasaaki Inaba
Aug 30, 2008·HSS Journal : the Musculoskeletal Journal of Hospital for Special Surgery·Safdar N KhanAaron Daluiski
Jan 14, 2012·Biointerphases·Mary E BoggsThomas P Beebe
Aug 2, 2011·Bone·Lindsay A BonsignoreEdward M Greenfield
Jul 6, 2010·Biochemical and Biophysical Research Communications·Hanguo WangHideaki Suda
Mar 14, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Harish K DattaR Dermot G Neely
Dec 11, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Brian L FosterMartha J Somerman
Aug 4, 2006·European Journal of Clinical Investigation·B Bielesz
Apr 3, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Andrew P SprowsonMark A Birch
Jul 29, 2010·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Claire BardetJean-Yves Sire
Sep 16, 2008·Biochemical and Biophysical Research Communications·Nan SuLin Chen
Apr 22, 2008·Archives of Biochemistry and Biophysics·Brendon S Noble
Jul 27, 2007·Journal of Dental Research·N SixM Goldberg
Jan 21, 2011·Journal of Dental Research·H G WangH Suda
Jun 16, 2012·The Journal of Endocrinology·Katherine A StainesColin Farquharson
Feb 2, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mingyue LiuWeiping Hu
Oct 5, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chuanyong LuRalph S Marcucio
Sep 21, 2017·Experimental and Therapeutic Medicine·Xianhua MengXiaoxia Liu
Mar 17, 2010·Expert Review of Endocrinology & Metabolism·Emily G Farrow, Kenneth E White
Apr 26, 2021·Pediatric Research·Hanna DanielssonAnn Hellström
Aug 8, 2021·International Journal of Molecular Sciences·Icela Palma-LaraCarmen Palacios-Reyes

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