PMID: 9180026May 5, 1997Paper

Meprobamate overdosage: a continuing problem. Sensitive GC-MS quantitation after solid phase extraction in 19 fatal cases

Forensic Science International : Synergy
Y GaillardG Pépin

Abstract

We describe a simple method for the urinary identification and blood quantitation of meprobamate suitable for any toxicological laboratory. After urinary screening using GC-MS technology, quantitation is performed by GC-MS in the selected-ion monitoring mode. Isolation of the drug is achieved by solid phase extraction on a C-18 cartridge. A specific elution is obtained by three volumes of acetone:triethylamine (99:1 v/v). Lidocaine is used as internal standard. RSDs (%) of the within-day and between-day precision studies are always less than 7.2 on the entire range of calibration. Linearity is inspected using an analysis of variance ANOVA. Homogeneity of the variances is tested using Hartley's test. Weighted linear regression is then computed. Limits of detection and quantification are given by an analysis of the blanks. The present method was applied in our laboratory over a period of 1 year. Meprobamate appeared as a drug which still has a significant frequency (5.5%) and is the most frequently involved in fatal pharmaceutical overdoses (15.3%). Post mortem concentrations ranged from 41 to 397 mg/l (mean = 182) and are compared to those of the literature.

References

Mar 1, 1985·Human Toxicology·J DennisonG N Volans
Jun 2, 1993·Journal of Chromatography·T TrenqueH Choisy

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Citations

Jun 27, 2008·International Journal of Legal Medicine·L FantonD Malicier
Feb 22, 2011·International Journal of Legal Medicine·Fabien BévalotLaurent Fanton
Mar 1, 2005·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Jørgen G BramnessDag Jacobsen
Jul 16, 2014·The International Journal on Drug Policy·Anita P Hardon, Amelia Ihsan
Dec 28, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xavier DelavenneThierry Basset
Nov 8, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jean-Claude AlvarezStanislas Grassin-Delyle
Oct 22, 2015·Journal of Analytical Toxicology·Giovanna TassoniMariano Cingolani
Feb 13, 2013·International Journal of Legal Medicine·F BévalotD Malicier
Jun 29, 1999·Analytical Chemistry·T A BrettellR Saferstein

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