Merkel cell polyomavirus-infected Merkel cell carcinoma cells require expression of viral T antigens

Journal of Virology
Roland HoubenJürgen Christian Becker


Merkel cell carcinoma (MCC) is the most aggressive skin cancer. Recently, it was demonstrated that human Merkel cell polyomavirus (MCV) is clonally integrated in approximately 80% of MCC tumors. However, direct evidence for whether oncogenic viral proteins are needed for the maintenance of MCC cells is still missing. To address this question, we knocked down MCV T-antigen (TA) expression in MCV-positive MCC cell lines using three different short hairpin RNA (shRNA)-expressing vectors targeting exon 1 of the TAs. The MCC cell lines used include three newly generated MCV-infected cell lines and one MCV-negative cell line from MCC tumors. Notably, all MCV-positive MCC cell lines underwent growth arrest and/or cell death upon TA knockdown, whereas the proliferation of MCV-negative cell lines remained unaffected. Despite an increase in the number of annexin V-positive, 7-amino-actinomycin D (7-AAD)-negative cells upon TA knockdown, activation of caspases or changes in the expression and phosphorylation of Bcl-2 family members were not consistently detected after TA suppression. Our study provides the first direct experimental evidence that TA expression is necessary for the maintenance of MCV-positive MCC and that MCV is the infecti...Continue Reading


Dec 19, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Mengtao LiYi-Ling Lin
Aug 21, 2013·The Journal of Laryngology and Otology·V BalakrishnanA Sizeland
Nov 26, 2010·Nature Reviews. Cancer·Patrick S Moore, Yuan Chang
Oct 15, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·Jürgen Christian Becker
Jan 12, 2012·Current Opinion in Oncology·David SchramaJürgen Christian Becker
May 11, 2012·Science Translational Medicine·Reety AroraPatrick S Moore
Jan 21, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Valentina NardiDora Dias-Santagata
Aug 16, 2011·The Journal of Clinical Investigation·Masahiro ShudaPatrick S Moore
Nov 2, 2012·The Journal of Clinical Investigation·Scott J RodigJames A DeCaprio
Oct 8, 2013·PLoS Computational Biology·Sven-Eric SchelhornFrank Berthold
Jul 30, 2011·PloS One·Huichen FengPatrick S Moore
Mar 1, 2012·PloS One·Christian HafnerJuergen C Becker
Aug 4, 2012·PloS One·Rachel M SchowalterChristopher B Buck
Jan 30, 2014·PloS One·Roland HoubenSebastian Haferkamp
Apr 4, 2014·PloS One·Christian AdamRoland Houben
Aug 2, 2011·Emerging Infectious Diseases·Yanis L TolstovYuan Chang
Nov 13, 2012·Bioinformation·Kazi Muhammad Ahasanul HoqueTonmay Modak Shuvra
Dec 15, 2010·International Journal of Biological Sciences·Zhi-Ming Zheng
Feb 1, 2014·Journal of Neurovirology·Kari WiedingerSulie Chang
Mar 12, 2013·Journal of Skin Cancer·Laura Desch, Rainer Kunstfeld
Jun 14, 2013·Case Reports in Oncological Medicine·Ahmed Abu-ZaidTarek Amin
Jun 4, 2015·Emerging Microbes & Infections·Masahiko Ajiro, Zhi-Ming Zheng
Jan 11, 2014·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Patrick J CiminoEric J Duncavage
Sep 29, 2011·Annual Review of Pathology·Yuan Chang, Patrick S Moore
Mar 23, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kelly G PaulsonPaul Nghiem
Aug 10, 2013·Wiener klinische Wochenschrift·Claudia LillDietmar Thurnher
Sep 19, 2015·International Journal of Cancer. Journal International Du Cancer·David SchramaRoland Houben
Jul 21, 2016·Iranian Red Crescent Medical Journal·Rouhollah VahabpourSeyed Hamidreza Monavari
Oct 25, 2014·Viruses·Abigail Morales-Sánchez, Ezequiel M Fuentes-Pananá


Jan 3, 1995·International Journal of Cancer. Journal International Du Cancer·J Helen LeonardJ R Bell
Nov 1, 1993·Journal of the American Academy of Dermatology·S G RonanT K Das Gupta
Feb 21, 2002·Lancet·Eric A EngelsRobert W Miller
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Mireille Van GeleFrank Speleman
Dec 22, 2004·Journal of Surgical Oncology·Nicole C Hodgson
Jul 15, 2005·Nature Medicine·Guido Kroemer, Seamus J Martin
Nov 22, 2005·Oncogene·Jason D Arroyo, William C Hahn
Jan 13, 2006·International Journal of Cancer. Journal International Du Cancer·Donald Maxwell Parkin
Sep 12, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Danielle L Poulin, James A DeCaprio
Jan 19, 2008·Science·Huichen FengPatrick S Moore
Feb 19, 2008·Journal of the American Academy of Dermatology·Michelle HeathPaul Nghiem
Jul 18, 2008·The Journal of Investigative Dermatology·Jürgen Christian BeckerDavid Schrama
Jul 25, 2008·The Journal of Investigative Dermatology·Kelly M GarneskiPaul Nghiem
Jul 25, 2008·The Journal of Investigative Dermatology·Katie RiddBoris C Bastian
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Masahiro ShudaYuan Chang
Nov 22, 2008·Cellular and Molecular Life Sciences : CMLS·Jürgen Christian BeckerRoland Houben
Mar 28, 2009·PLoS Pathogens·Jaime M KeanRobert L Garcea
Apr 23, 2009·International Journal of Cancer. Journal International Du Cancer·Ahmad KassemAxel Zur Hausen
Apr 30, 2009·Experimental Dermatology·Roland HoubenJürgen Christian Becker
Jun 6, 2009·International Journal of Cancer. Journal International Du Cancer·Masahiro ShudaYuan Chang
Jun 6, 2009·International Journal of Cancer. Journal International Du Cancer·Yanis L TolstovPatrick S Moore
Jun 10, 2009·Seminars in Cancer Biology·Jingwei ChengBrian S Schaffhausen
Jun 19, 2009·Journal of the National Cancer Institute·Harri SihtoHeikki Joensuu
Jun 30, 2009·Molecular Oncology·Andreas BrechHarald Stenmark
Sep 15, 2009·PLoS Pathogens·Diana V PastranaChristopher B Buck
Sep 25, 2009·Journal of the National Cancer Institute·Joseph J CarterDenise A Galloway
Oct 8, 2009·International Journal of Cancer. Journal International Du Cancer·Emilie LanoyEric A Engels

Related Concepts

Malignant Neoplasm of Skin
Annexin A5
Gene Knockdown Techniques
Viral Tumor Antigens
Viral Proteins
BCL2 gene
Protein Phosphorylation
ANXA5 gene

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.