Mesenchymal-epithelial transition in epithelial response to injury: the role of Foxc2.

Oncogene
C HaderL Cantley

Abstract

Overexpression of the forkhead family transcription factor Foxc2 has been shown to activate epithelial-mesenchymal transition (EMT) and correlate with tumor metastasis. In this study, we show that both mRNA and protein levels of Foxc2 increase 1 day after kidney ischemia/reperfusion in sublethally injured tubular cells and that the protein is located in the cytoplasm rather than the nucleus of these cells. in vitro studies of cultured tubular cells confirm the cytoplasmic location of Foxc2 and show that increased cytoplasmic expression of Foxc2 correlates with epithelial differentiation rather than dedifferentiation. Silencing of Foxc2 by RNAi in these cells led to EMT and increased cell migration. In contrast, Foxc2 is found in both the nucleus and cytoplasm of cultured fibroblasts, with RNAi leading to increased expression of epithelial markers and impaired cell migration. Consistent with a subcellular localization dependence of Foxc2 function, overexpression of Foxc2 in renal epithelial cells resulted in de novo nuclear expression of the protein and promotion of a mesenchymal/fibroblast phenotype. These results suggest that Foxc2 may have regulatory functions independent of its nuclear transcriptional activity and that upreg...Continue Reading

References

Jul 1, 1995·The Journal of Cell Biology·F StrutzE G Neilson
Nov 15, 1997·The American Journal of Physiology·J A SchaferE Schlatter
Nov 7, 2002·American Journal of Physiology. Renal Physiology·Diviya SinhaWilfred Lieberthal
Feb 28, 2003·The EMBO Journal·Philippe R J Bois, Gerard C Grosveld
May 23, 2003·Journal of the American Society of Nephrology : JASN·Joseph V Bonventre
Jun 26, 2003·The Journal of Clinical Investigation·Sujata KaleLloyd G Cantley
Dec 18, 2003·The Journal of Clinical Investigation·Raghu Kalluri, Eric G Neilson
Mar 3, 2004·Nature Reviews. Molecular Cell Biology·Lan Xu, Joan Massagué
Nov 2, 2004·Nature Reviews. Immunology·Paul J Coffer, Boudewijn M T Burgering
Jul 12, 2005·The Journal of Clinical Investigation·Fangming LinPeter Igarashi
Sep 1, 2005·Apoptosis : an International Journal on Programmed Cell Death·T S SchwabE L Feldman
Jun 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sendurai A ManiRobert A Weinberg
Oct 19, 2007·Nature Reviews. Cancer·Stephen S Myatt, Eric W-F Lam
Jan 12, 2008·Biochemical and Biophysical Research Communications·Hisaki Hayashi, Tsutomu Kume
Apr 9, 2008·Oncogene·D R Calnan, A Brunet
Jun 27, 2008·The Journal of Biological Chemistry·Hisaki HayashiTsutomu Kume
Jul 29, 2008·Current Opinion in Nephrology and Hypertension·Shuta Ishibe, Lloyd G Cantley
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jonathan B L BardStuart Aitken

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Citations

Dec 17, 2011·Journal of Oncology·Tsutomu Kume
Jul 3, 2013·BMC Musculoskeletal Disorders·Wulin YouKunzheng Wang
May 30, 2013·PloS One·Aurélie Allais-BonnetAurélien Capitan
Jan 8, 2013·Journal of the American Society of Nephrology : JASN·Insa M SchmidtLloyd G Cantley
Mar 1, 2012·World Journal of Gastroenterology : WJG·Ferenc Sipos, Orsolya Galamb
Aug 31, 2010·Annals of Surgical Oncology·Naohiro NishidaMasaki Mori
Jul 15, 2015·International Journal of Oncology·Wen-Long ZhangWei Lv
Jun 15, 2011·Birth Defects Research. Part C, Embryo Today : Reviews·Lisa M SwanhartNeil A Hukriede
Jan 5, 2011·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Marwa El Sabbahy, Vishal S Vaidya
May 16, 2012·Cell Biology International·Patrick C Baer, Jürgen Bereiter-Hahn
Jul 28, 2011·Journal of Cellular Physiology·R ThalerF Varga
May 21, 2015·Cellular and Molecular Life Sciences : CMLS·Odile FilholClaude Cochet
Jun 10, 2017·Acta Obstetricia Et Gynecologica Scandinavica·Masataka FuruyaHideyuki Saya
Nov 26, 2015·Molecular and Cellular Biology·Laura WiehleAchim Breiling
Sep 17, 2010·American Journal of Physiology. Renal Physiology·Xiaohua WangJunwei Yang
Feb 13, 2017·Archives of Gynecology and Obstetrics·Magdalena MatysiakMarcin Kruszewski
May 31, 2018·International Journal of Molecular Medicine·Xiaoxu GaoRuifang An
Mar 11, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Quiana McKnightJackie A Fretz
Dec 11, 2020·Cells·Yun Kyung LeeJun Hong Park

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Methods Mentioned

BETA
transfection
electrophoresis
PCR
laser capture microdissection
dissection

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