Mesenchymal stem cell-based angiopoietin-1 gene therapy for acute lung injury induced by lipopolysaccharide in mice

The Journal of Pathology
Jianguo XuL Yu

Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) can serve as a vehicle for gene therapy. Angiopoietin-1 (Ang1) is a critical factor for endothelial survival and vascular stabilization via the inhibition of endothelial permeability and leukocyte-endothelium interactions. We hypothesized that MSC-based Ang1 gene therapy might be a potential therapeutic approach for lipopolysaccharide (LPS)-induced lung injury. MSCs were isolated from 6 week-old inbred male mice and transduced with the Ang1 gene, using a lentivirus vector. The MSCs showed no significant phenotypic changes after transduction. In the in vivo mouse model, the LPS-induced lung injury was markedly alleviated in the group treated with MSCs carrying Ang1 (MSCs-Ang1), compared with groups treated with MSCs or Ang1 alone. The expression of Ang1 protein in the recipient lungs was increased after MSCs-Ang1 administration. The histopathological and biochemical indices of LPS-induced lung injury were improved after MSCs-based Ang1 gene treatment. MSCs-Ang1 administration also reduced pulmonary vascular endothelial permeability and the recruitment of inflammatory cells into the lung. Cells of MSC origin could be detected in the recipient lungs for 2 weeks after injection with...Continue Reading

References

Jul 27, 2001·American Journal of Respiratory Cell and Molecular Biology·E P IngenitoL A Sonna
Dec 1, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Injune KimGou Young Koh
Feb 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hideki HanawaArthur W Nienhuis
Aug 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dimitrios KarmpaliotisKathleen J Haley
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey L SpeesDarwin J Prockop
Mar 29, 2003·American Journal of Respiratory and Critical Care Medicine·Michael A MatthayAndrea L Harabin
May 29, 2003·British Journal of Pharmacology·Lara PizurkiAndreas Papapetropoulos
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Luis A OrtizDonald G Phinney
Aug 2, 2003·Blood·Erica L HerzogDiane S Krause
Aug 9, 2003·The New England Journal of Medicine·Martin Kørbling, Zeev Estrov
Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mitsuhiro YamadaHidetada Sasaki
Jan 27, 2004·The Journal of Pathology·Madhav Bhatia, Shabbir Moochhala
Apr 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chung-Hyun ChoGou Young Koh
Oct 21, 2004·Journal of Cell Science·Fernando Anjos-AfonsoDominique Bonnet
Nov 4, 2004·Journal of the National Cancer Institute·Matus StudenyMichael Andreeff
Nov 24, 2004·Infection and Immunity·Samithamby JeyaseelanG Scott Worthen
Dec 22, 2004·Circulation·Bernhard WitzenbichlerCarsten Tschope
Jan 25, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jeffrey R JacobsonJoe G N Garcia
Feb 25, 2005·Anesthesia and Analgesia·Yumiko TakaoHidefumi Obara
May 14, 2005·American Journal of Respiratory Cell and Molecular Biology·Mauricio RojasKenneth L Brigham
Jun 18, 2005·American Journal of Respiratory Cell and Molecular Biology·Darrell N KottonRichard C Mulligan
Sep 24, 2005·Gene Therapy·J Polak, L Hench
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Roberto LoiDaniel J Weiss
Dec 2, 2005·Expert Opinion on Biological Therapy·Jakob ReiserVincent F La Russa
Mar 2, 2006·The European Respiratory Journal·J A Denburg, S F van Eeden
Apr 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Barry R Stripp, Steven D Shapiro
Feb 27, 2007·American Journal of Respiratory and Critical Care Medicine·Sarah D McCarterDuncan J Stewart
Jun 15, 2007·The Journal of Biological Chemistry·Tadanori MammotoVikas P Sukhatme
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naveen GuptaMichael A Matthay

❮ Previous
Next ❯

Citations

Jun 29, 2011·Molecular Pharmaceutics·Hao WuRam I Mahato
Sep 16, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ahmed M KatshaYasuo Saijo
Mar 8, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Allison N LauDaniel J Weiss
Mar 10, 2012·Antioxidants & Redox Signaling·Rajesh S AlphonseBernard Thébaud
Jul 5, 2011·British Medical Bulletin·Philipp Jungebluth, Paolo Macchiarini
Jan 5, 2011·Critical Care Medicine·Michael A MatthayJae Woo Lee
Sep 15, 2010·Critical Care Medicine·Sushma K CribbsGreg S Martin
Mar 16, 2011·Clinical and Experimental Pharmacology & Physiology·Jin-Fu XuHui-Ping Li
Jan 5, 2011·Respirology : Official Journal of the Asian Pacific Society of Respirology·Yuben MoodleyDaniel J Weiss
Apr 16, 2009·Clinical Microbiology Reviews·Ineke Vanlaere, Claude Libert
Jun 5, 2010·American Journal of Respiratory and Critical Care Medicine·Yuben MoodleyUrsula Manuelpillai
Jan 8, 2013·American Journal of Respiratory and Critical Care Medicine·Jae W LeeMichael A Matthay
May 10, 2013·American Journal of Respiratory Cell and Molecular Biology·Itziar Martínez-GonzálezJosep M Aran
Jul 16, 2008·Proceedings of the American Thoracic Society·Daniel J WeissDarwin J Prockop
Jun 10, 2011·Proceedings of the American Thoracic Society·Daniel J WeissDarwin J Prockop
Feb 25, 2014·American Journal of Respiratory and Critical Care Medicine·Martha L BustosMauricio Rojas
Jul 26, 2011·Mediators of Inflammation·Ying-Gang ZhuJin-Fu Xu
Dec 10, 2008·Expert Opinion on Therapeutic Targets·Melanie van der HeijdenA B Johan Groeneveld
Dec 7, 2010·Expert Review of Respiratory Medicine·Hong ChenXiangdong Wang
Dec 7, 2010·Expert Review of Respiratory Medicine·Darryl A KnightTillie-Louise Hackett
Aug 21, 2009·Expert Opinion on Biological Therapy·Jae Woo LeeMichael A Matthay
Jan 22, 2011·Stem Cells International·Beth A DrzewieckiStacy T Tanaka
Jan 17, 2009·International Immunopharmacology·Patricia SemedoNiels Olsen Saraiva Câmara
Jun 13, 2015·American Journal of Respiratory and Critical Care Medicine·Antoine MonselJae W Lee
Apr 4, 2015·Stem Cells and Development·Jing LiHong-Gang Duan
Aug 13, 2015·BMC Medicine·Naomi D'souzaMassimo Dominici

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