Mesenchymal stem cell-conditioned medium improves the proliferation and migration of keratinocytes in a diabetes-like microenvironment

The International Journal of Lower Extremity Wounds
Meirong LiXiaobing Fu

Abstract

The impairment of wound healing in diabetic patients is an important clinical problem. Proper keratinocyte migration and proliferation are the crucial steps during reepithelialization, and these steps may be impaired in diabetes mellitus (DM) due to hyperglycemia and chronic inflammation in wound site. In this study, we explored the effects of diabetes-like microenvironment with high glucose (HG) and intense inflammation on the migration and proliferation of keratinocytes in vitro. We found that the migration and proliferation of rat keratinocytes were reduced with HG and lipopolysaccharide (LPS) stimulation via Erk signaling pathway in a reactive oxygen species (ROS)-dependent manner. Nevertheless, mesenchymal stem cell-conditioned medium (MSC-CM) counteracts the effects of HG and LPS. Treatment of rat keratinocyte with MSC-CM decreased HG- and/or LPS-induced ROS overproduction. Furthermore, MSC-CM reversed the downregulation of phosphorylation of MEK1/2 and Erk 1/2, which was induced by HG and/or LPS without affecting total levels. Our results may provide a possible mechanism for delayed wound healing in DM and provide a foundation to develop MSC-CM as an alternative therapeutic strategy to ameliorate the poor wound-healing c...Continue Reading

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Citations

Jan 16, 2016·Stem Cells International·Deyun ChenWeidong Han
Jun 22, 2017·The Annals of Otology, Rhinology, and Laryngology·Nao HiwatashiRyan C Branski
Feb 15, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ramla Sana KhalidAsmat Salim
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Mar 7, 2021·International Journal of Molecular Sciences·Hantae JoBum-Ho Bin
Oct 9, 2021·Scientific Reports·Bartosz SikoraJoanna Gola

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Methods Mentioned

BETA
fluorescence
flow cytometry
FACS
Polymerase Chain Reaction
PCR
electrophoresis
scraping
confocal microscopy
fluorescence microscopy

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