Mesenchymal stem cell content of human vertebral bone marrow

Transplantation
Norbert AhrensStefan Scheding

Abstract

Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably higher than in iliac crest-BM (1.4/10(5) NC, range: 0.4-2.6) and peripheral blood progenitor cells (not detectable). MSC generated from V-BM had the typical MSC phenotype (CD105(pos)CD73(pos)CD45(neg)CD34(neg)), displayed multilineage differentiation potential, and suppressed alloreactivity in mixed lymphocyte reactions. V-BM may be an excellent source for MSC cotransplantation approaches.

References

Aug 15, 1998·Vox Sanguinis·S SerkeD Huhn
Mar 30, 2001·Bone Marrow Transplantation·O N Koç, H M Lazarus
Sep 7, 2001·Current Opinion in Immunology·H Waldmann
Sep 20, 2002·Blood·Alois GratwohlUNKNOWN Accreditation Committee of the European Group for Blood and Marrow Transplantation (EBMT)

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Citations

Oct 30, 2013·Der Urologe. Ausg. A·M VaeglerK-D Sievert
May 14, 2010·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Woo-Kie MinShin-Yoon Kim
Oct 6, 2009·Bone Marrow Transplantation·S S KellyE Shpall
Sep 15, 2010·Best Practice & Research. Clinical Haematology·S S TungE J Shpall
Mar 4, 2010·Cytotherapy·Susan Staba KellyElizabeth Shpall
Sep 28, 2007·British Journal of Haematology·Richard Veyrat-MassonMarc G Berger
Sep 7, 2007·Journal of Biomedical Materials Research. Part a·Toshihiro SugiokaShinobu Yanada
Sep 30, 2010·International Journal of Cancer. Journal International Du Cancer·Jan C BruneStefan Scheding
Feb 16, 2011·Journal of Cellular Biochemistry·Carola CavalloLorenzo Moroni
Aug 6, 2005·Cytotherapy·S RobinsonE Shpall
Aug 8, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Jacek ToporskiAlbert N Békássy
Jun 9, 2016·Annals of the New York Academy of Sciences·Hongzhe LiStefan Scheding
Nov 2, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bruno C MenarimLinda A Dahlgren
Aug 8, 2020·Journal of Translational Medicine·Erik J WoodsBrian H Johnstone
Feb 9, 2020·Stem Cell Research & Therapy·Afsoon AfshariNegar Azarpira
Nov 21, 2007·Cell Transplantation·Mustapha NajimiEtienne Marc Sokal

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