Mesenchymal stem cell-derived exosomes improve motor function and attenuate neuropathology in a mouse model of Machado-Joseph disease.

Stem Cell Research & Therapy
Hua-Jing YouZhong Pei

Abstract

Machado-Joseph disease is the most common autosomal dominant hereditary ataxia worldwide without effective treatment. Mesenchymal stem cells (MSCs) could slow the disease progression, but side effects limited their clinical application. Besides, MSC-derived exosomes exerted similar efficacy and have many advantages over MSCs. The aim of this study was to examine the efficacy of MSC-derived exosomes in YACMJD84.2 mice. Rotarod performance was evaluated every 2 weeks after a presymptomatic administration of intravenous MSC-derived exosomes twice in YACMJD84.2 mice. Loss of Purkinje cells, relative expression level of Bcl-2/Bax, cerebellar myelin loss, and neuroinflammation were assessed 8 weeks following treatment. MSC-derived exosomes were isolated and purified through anion exchange chromatography. Better coordination in rotarod performance was maintained for 6 weeks in YACMJD84.2 mice with exosomal treatment, compared with those without exosomal treatment. Neuropathological changes including loss of Purkinje cells, cerebellar myelin loss, and neuroinflammation were also attenuated 8 weeks after exosomal treatment. The higher relative ratio of Bcl-2/Bax was consistent with the attenuation of loss of Purkinje cells. MSC-derived ...Continue Reading

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Citations

Dec 15, 2020·Journal of Chromatography. a·Thanaporn LiangsupreeMarja-Liisa Riekkola
Aug 23, 2021·Advanced Drug Delivery Reviews·Simon StaubachBernd Giebel
Nov 10, 2021·European Journal of Clinical Investigation·Catarina Oliveira Miranda

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Methods Mentioned

BETA
transgenic
PCR
Fluorescence
Protein Assay
transmission electron microscopy

Software Mentioned

ImageJ

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