Mesenchymal Stem Cell-Like Properties of Orbital Fibroblasts in Graves' Orbitopathy

Investigative Ophthalmology & Visual Science
Katarzyna KozdonMaryse Bailly

Abstract

Graves' orbitopathy (GO) is a sight-threatening autoimmune disorder causing extraocular muscle fibrosis, upper lid retraction and eye bulging due to orbital fat expansion. These clinical features are mediated by aspects of orbital fibroblasts differentiation, including adipogenesis and fibrosis. Our previous work suggested that this dual phenotype might be a manifestation of mixed cell populations, partially linked to the expression of mesenchymal stem cell (MSC) marker CD90. Thus, we set out to determine whether GO orbital fibroblasts displayed MSC properties. Control and GO orbital fibroblasts previously characterized for CD90 and CD45 expression were analyzed by flow cytometry for classical MSC positive (CD73, CD105) and negative (CD14, CD19, HLA-DR, and CD34) markers. Graves' orbitopathy fibroblasts were tested further for their ability to undergo lineage specific differentiation following standard protocols. Control and GO fibroblasts strongly expressed CD73 and CD105, with a higher percentage of positive cells and stronger expression levels in GO. Neither cell type expresses CD14, CD19, and HLA-DR. Protein CD34 was expressed at low levels by 45% to 70% of the cells, with its expression significantly lower in GO cells. Gra...Continue Reading

Citations

Jun 29, 2017·Journal of Cellular Physiology·Bhawna Chandravanshi, Ramesh Bhonde
Dec 8, 2017·Stem Cells International·Richárd NagymihályGoran Petrovski
Dec 15, 2018·Investigative Ophthalmology & Visual Science·Catherine J ChoiSara T Wester
Jan 1, 2020·Nature Reviews. Endocrinology·Peter N TaylorMarian Ludgate
Nov 11, 2019·Chemico-biological Interactions·Hong LiYali Zhang
Dec 4, 2020·International Journal of Molecular Sciences·Lei ZhangColin M Dayan
Nov 26, 2020·Ophthalmic Plastic and Reconstructive Surgery·Jonathan C P RoosRachna Murthy

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