Mesenchymal Stem Cell Secretome Enhancement by Nicotinamide and Vasoactive Intestinal Peptide: A New Therapeutic Approach for Retinal Degenerative Diseases

Stem Cells International
Maria L Alonso-AlonsoIvan Fernandez-Bueno

Abstract

Mesenchymal stem cells (MSC) secrete neuroprotective molecules that may be useful as an alternative to cell transplantation itself. Our purpose was to develop different pharmaceutical compositions based on conditioned medium (CM) of adipose MSC (aMSC) stimulated by and/or combined with nicotinamide (NIC), vasoactive intestinal peptide (VIP), or both factors; and to evaluate in vitro their proliferative and neuroprotective potential. Nine pharmaceutical compositions were developed from 3 experimental approaches: (1) unstimulated aMSC-CM collected and combined with NIC, VIP, or both factors (NIC+VIP), referred to as the aMSC-CM combined composition; (2) aMSC-CM collected just after stimulation with the mentioned factors and containing them, referred to as the aMSC-CM stimulated-combined composition; and (3) aMSC-CM previously stimulated with the factors, referred to as the aMSC stimulated composition. The potential of the pharmaceutical compositions to increase cell proliferation under oxidative stress and neuroprotection were evaluated in vitro by using a subacute oxidative stress model of retinal pigment epithelium cells (line ARPE-19) and spontaneous degenerative neuroretina model. Results showed that oxidatively stressed ARPE...Continue Reading

References

Sep 1, 1990·Experimental Eye Research·A P Swedlund, S A Rosenzweig
Feb 1, 1996·Experimental Eye Research·K C DunnL M Hjelmeland
Apr 29, 1998·In Vitro Cellular & Developmental Biology. Animal·S L Voytik-HarbinS F Badylak
Mar 5, 1999·Journal of Neuroscience Methods·J M OgilvieT T Fleming
Jun 22, 1999·Experimental Eye Research·S W BallingerB F Godley
Oct 18, 2000·Survey of Ophthalmology·S BeattyM Boulton
Jun 9, 2001·Veterinary Ophthalmology·M.J. ChandlerE.O. MacKay
Nov 24, 2001·Journal of Chemical Neuroanatomy·A R CafféT van Veen
Jun 22, 2002·Experimental Eye Research·Anita Hendrickson, David Hicks
May 15, 2003·Survey of Ophthalmology·Jayakrishna AmbatiAnthony P Adamis
Jul 29, 2003·Korean Journal of Ophthalmology : KJO·Min-Ho KimJin-Seong Yoo
Nov 12, 2003·The British Journal of Ophthalmology·J TrogerW Göttinger
Sep 24, 2005·Annals of the New York Academy of Sciences·Diamond TamKenneth I Maynard
Jul 28, 2007·Peptides·Olfa Masmoudi-KoukiMarie-Christine Tonon
Apr 15, 2008·Investigative Ophthalmology & Visual Science·Xinyu ZhangFu Shang
Jul 3, 2008·Methods : a Companion to Methods in Enzymology·Bruce A BunnellCynthia Ripoll
Oct 14, 2008·Progress in Retinal and Eye Research·Stuart G JarrettMichael E Boulton
Jan 27, 2010·Free Radical Biology & Medicine·Patrycja KaczaraJanice M Burke
Aug 21, 2010·Journal of Nucleic Acids·Devita SurjanaDiona L Damian
Sep 11, 2010·Ophthalmic Research·Stuart G JarrettMichael E Boulton
Mar 23, 2011·Journal of Translational Medicine·Carla A HerbertsHarm P H Hermsen
Apr 19, 2012·Molecular Aspects of Medicine·Stuart G Jarrett, Michael E Boulton
May 1, 2012·Journal of Molecular Neuroscience : MN·K SzabadfiD Reglodi
Jun 8, 2012·Drug Development and Industrial Pharmacy·Xu CuiAiping Zheng
Aug 23, 2012·Apoptosis : an International Journal on Programmed Cell Death·Yujuan WangChi-Chao Chan
Apr 29, 2014·World Journal of Stem Cells·Tsz Kin NgHerman S Cheung
Jul 20, 2014·Progress in Retinal and Eye Research·Nicolás CuencaIsabel Pinilla
Apr 29, 2015·World Journal of Stem Cells·María L Alonso-Alonso, Girish K Srivastava
Jun 27, 2015·Progress in Retinal and Eye Research·Elena VecinoSansar C Sharma
Jul 30, 2015·BMC Complementary and Alternative Medicine·Simon Bernard Iloki-AssangaJosé Luis Rubio-Pino
Aug 1, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Silvia J H ParkJonathan B Demb

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Citations

Apr 28, 2021·Stem Cell Reviews and Reports·Maria Luz Alonso-AlonsoYolanda Diebold

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