Mesenchymal stem cell therapy following muscle trauma leads to improved muscular regeneration in both male and female rats

Gender Medicine
Philipp von RothTobias Winkler

Abstract

Mesenchymal stem cell (MSC) therapy has the potential to enhance muscular regeneration. In previous publications, our group was able to show a dose-response relationship in female animals between the amount of transplanted cells and muscle force. The impact of sex on the regeneration of musculoskeletal injuries following MSC transplantation remains unclear. We investigated histologic and biomechanical regeneration parameters in rats after autologous transplantation of MSCs. Our hypothesis was that female rats have greater muscle regeneration potential than male rats after autologous MSC transplantation. Thirty-six Sprague-Dawley rats received an open crush trauma of the left soleus muscle. One week after trauma, 2.5 × 10(6) autologous MSCs, harvested from tibial biopsies, were transplanted locally (female, n = 9; male, n = 9). Control animals received saline solution (female, n = 9; male, n = 9). Histologic analysis and biomechanical evaluation by in vivo muscle force measurement were performed 3 weeks after transplantation. MSC therapy improved the force of the injured soleus in male rats significantly (twitch: treated, 0.76 [0.51-1.15]; twitch: untreated, 0.45 [0.32-0.73] [P = 0.01]; tetany: treated, 0.63 [0.4-1.21], tetany: ...Continue Reading

References

Jan 1, 1997·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·I B RáczJ Hamar
May 6, 2005·Molecular Biology of the Cell·B M DeasyJ Huard
Aug 5, 2005·Journal of Pharmacological Sciences·Ryotaro WakeHiroshi Iwao
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Masaya TakahashiKimikazu Hamano
Nov 26, 2008·Circulation Research·Massimiliano GnecchiVictor J Dzau
Jun 16, 2009·Cell Transplantation·Lauren DrowleyJohnny Huard
Aug 18, 2009·Experimental Neurology·Radbod DarabiRita C R Perlingeiro
Dec 15, 2010·Acta Orthopaedica·Tobias WinklerStephan V Tohtz
Jul 19, 2011·Gender Medicine·Israel DudkiewiczAvital Hershkovitz

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Citations

Sep 4, 2013·Regenerative Medicine·Benjamin M DaviesAndrew Carr
Aug 31, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Rongguo WangYunting Wang
Mar 9, 2017·International Journal of Molecular Sciences·Kabilan ThurairajahZsolt J Balogh
Jun 7, 2014·Medicine and Science in Sports and Exercise·Kai ZouMarni D Boppart
Mar 14, 2018·International Journal of Molecular Sciences·F Andrea SassKatharina Schmidt-Bleek
May 19, 2018·Journal of Tissue Engineering and Regenerative Medicine·Marissa A RuehleJarrod A Call
Nov 30, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Tie-Shan LiChuanzhu Yan
Nov 3, 2020·Biological & Pharmaceutical Bulletin·Yusuke KonoTakuya Fujita

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