Mesenchymal Stem Cells Alleviate LPS-Induced Acute Lung Injury in Mice by MiR-142a-5p-Controlled Pulmonary Endothelial Cell Autophagy

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Zichao Zhou, Zhijian You

Abstract

Damages of pulmonary endothelial cells (PECs) represent a critical pathological process during acute lung injury (ALI), and precede pulmonary epithelial cell injury, and long-term lung dysfunction. Transplantation of mesenchymal stem cells (MSCs) has proven therapeutic effects on ALI, whereas the underlying mechanisms remain ill-defined. We transplanted MSCs in mice and then induced ALI using Lipopolysaccharides (LPS). We analyzed the changes in permeability index and lung histology. Mouse PECs were isolated by flow cytometry based on CD31 expression and then analyzed for autophagy-associated factors LC3 and Beclin-1 by Western blot. Beclin-1 mRNA was determined by RT-qPCR. In vitro, we performed bioinformatics analyses to identify the MSCs-regulated miRNAs that target Beclin-1, and confirmed that the binding was functional by 3'-UTR luciferase reporter assay. We found that MSCs transplantation significantly reduced the severity of LPS-induced ALI in mice. MSCs increased autophagy of PECs to promote PEC survival. MSCs increased Beclin-1 protein but not mRNA. MiR-142a-5p was found to target the 3'-UTR of Beclin-1 mRNA to inhibit its protein translation in PECs. MSCs reduced the levels of miR-142a-5p in PECs from LPS-treated mice...Continue Reading

Citations

Dec 23, 2017·Journal of Cellular Physiology·Yuqing ZhuAhmed El-Hashash
Jan 4, 2018·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Hualiang LiZhenhua Ding
Feb 16, 2018·BioMed Research International·Shuming PanChengjin Gao
Mar 21, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qing-Chun LiZhen-Bo Su
Sep 12, 2019·Frontiers in Physiology·Xin YinLiheng Guo
Sep 17, 2020·Stem Cell Research & Therapy·Fatemeh MirershadiRana Keyhanmanesh
Jun 30, 2018·Molecular Medicine Reports·Xun WangXiao-Lei Shi
Dec 14, 2018·Experimental and Therapeutic Medicine·Wei-Dong ZhuKe Sun
Oct 11, 2019·Precision Clinical Medicine·Qinqin PuMin Wu
Aug 24, 2020·Life Sciences·Selvaraj VishnupriyaRajan Radha Rasmi
Apr 2, 2021·Life Sciences·Yuexian LiJin Zhang

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