Mesenchymal Stem Cells and Mononuclear Cells From Cord Blood: Cotransplantation Provides a Better Effect in Treating Myocardial Infarction

Stem Cells Translational Medicine
Gecai ChenLi Zhu

Abstract

The aim of this study was to evaluate the effect of cotransplanting mononuclear cells from cord blood (CB-MNCs) and mesenchymal stem cells (MSCs) as treatment for myocardial infarction (MI). Transplanting CD34+ cells or MSCs separately has been shown effective in treating MI, but the effect of cotransplanting CB-MNCs and MSCs is not clear. In this study, MSCs were separated by their adherence to the tissue culture. The morphology, immunophenotype, and multilineage potential of MSCs were analyzed. CB-MNCs were separated in lymphocyte separation medium 1.077. CD34+ cell count and viability were analyzed by flow cytometry. Infarcted male Sprague-Dawley rats in a specific-pathogen-free grade were divided into four treatment groups randomly: group I, saline; group II, CB-MNCs; group III, MSCs; and group IV, CB-MNCs plus MSCs. The saline, and CB-MNCs and/or MSCs were injected intramyocardially in infarcted rats. Their cardiac function was evaluated by echocardiography. The myocardial capillary density was analyzed by immunohistochemistry. Both cell types induced an improvement in the left ventricular cardiac function and increased tissue cell proliferation in myocardial tissue and neoangiogenesis. However, CB-MNCs plus MSCs were more...Continue Reading

References

Apr 14, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Jaap Jan BoelensUNKNOWN EUROCORD, Inborn error Working Party of EBMT and Duke University
May 15, 2010·Journal of the American College of Cardiology·Ana ArmiñánPilar Sepúlveda
Jun 26, 2010·International Journal of Hematology·Myoung Woo LeeHong Hoe Koo
Jan 5, 2011·Journal of Cellular and Molecular Medicine·Zhuzhi WenTong Wang
Apr 12, 2011·International Journal of Hematology·Hiroshi YagasakiHideo Mugishima
May 17, 2011·British Journal of Haematology·Tomohiro MorioUNKNOWN Japanese Cord Blood Bank Network
Dec 21, 2011·International Journal of Hematology·Lei HaoZhongmin Zou
Jul 9, 2013·Experimental Hematology·Hélène Labussière WalletMauricette Michallet
Nov 1, 2008·International Journal of Stem Cells·Il-Kwon Ko, Byung-Soo Kim
Jul 1, 2014·Journal of Cellular and Molecular Medicine·Qingfeng MiaoPhilip Wong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.

© 2022 Meta ULC. All rights reserved