Mesenchymal Stem Cells Attenuate Radiation-Induced Brain Injury by Inhibiting Microglia Pyroptosis

BioMed Research International
Huan LiaoYing Peng

Abstract

Radiation-induced brain injury (RI) commonly occurs in patients who received head and neck radiotherapy. However, the mechanism of RI remains unclear. We aimed to evaluate whether pyroptosis was involved in RI and the impact of mesenchymal stem cells (MSCs) on it. BALB/c male mice (6-8 weeks) were cranially irradiated (15 Gy), and MSCs were transplanted into the bilateral cortex 2 days later; then mice were sacrificed 1 month later. Meanwhile, irradiated BV-2 microglia cells (10 Gy) were cocultured with MSCs for 24 hours. We observed that irradiated mice brains presented NLRP3 and caspase-1 activation. RT-PCR then indicated that it mainly occurred in microglia cells but not in neurons. Further, irradiated BV-2 cells showed pyroptosis and increased production of IL-18 and IL-1β. RT-PCR also demonstrated an increased expression of several inflammasome genes in irradiated BV-2 cells, including NLRP3 and AIM2. Particularly, NLRP3 was activated. Knockdown of NLRP3 resulted in decreased LDH release. Noteworthily, in vivo, MSCs transplantation alleviated radiation-induced NLRP3 and caspase-1 activation. Moreover, in vitro, MSCs could decrease caspase-1 dependent pyroptosis, NLRP3 inflammasome activation, and ROS production induced by ...Continue Reading

References

Aug 18, 2000·International Journal of Radiation Biology·U RajuP J Tofilon
Apr 23, 2002·The Journal of Biological Chemistry·Srinivasa M SrinivasulaEmad S Alnemri
Oct 15, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T B JohannesenK Lote
Oct 6, 2005·Neurobiology of Disease·So-Young HwangInn-Oc Han
Jan 11, 2007·The British Journal of Radiology·M GencD A Kiresi
Apr 15, 2008·International Journal of Radiation Oncology, Biology, Physics·Jing LiMinesh P Mehta
May 2, 2008·International Journal of Radiation Oncology, Biology, Physics·Grit WelzelFrederik Wenz
Dec 25, 2008·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Grit WelzelFrederik Wenz
Nov 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Munjal M AcharyaCharles L Limoli
Mar 23, 2010·Cell·Kate Schroder, Jurg Tschopp
Nov 16, 2010·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Chris BeltranThomas E Merchant
Sep 3, 2011·Immunological Reviews·Edward A MiaoAlan Aderem
Feb 8, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dana Greene-SchloesserMike E Robbins
Sep 26, 2013·International Journal of Radiation Oncology, Biology, Physics·Ansa Maer ColeDiana Steinmann
Oct 18, 2014·Nature Reviews. Immunology·Andreas LinkermannHans-Joachim Anders
Nov 13, 2014·Journal of Pineal Research·Francisco OrtizGermaine Escames
Dec 30, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veit M StoeckleinJames A Lederer
Mar 26, 2015·Environmental Toxicology and Pharmacology·Sung-Hwa SohnHyunsu Bae
May 21, 2015·American Society of Clinical Oncology Educational Book·Minesh P Mehta, Manmeet S Ahluwalia
Apr 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Janet E BaulchCharles L Limoli
Oct 27, 2016·Brain and Behavior·Yogi Chang-Yo HsuanMao-Tsun Lin
Nov 5, 2016·Experimental & Molecular Medicine·Jongdoo KimJong-Sup Bae

❮ Previous
Next ❯

Citations

Jan 15, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Faqin LiangWei Wei
Aug 29, 2020·Oxidative Medicine and Cellular Longevity·Liren Qian, Jian Cen
Jun 18, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Maha Mohammed Abdel-FattahAhmed Mohamed Mansour
Sep 19, 2020·International Journal of Radiation Biology·Eloise ParisetSylvain V Costes
Nov 5, 2020·Biomedicines·Elena ObradorAlegría Montoro
Jun 5, 2021·Frontiers in Pharmacology·Lalitha Sarad Yamini NanduriChandan Guha

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
ELISAs
Transfections
transfection
confocal microscopy
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Related Papers

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Juan Pablo de Rivero VaccariRobert W Keane
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
Yan WangQiang Liu
© 2022 Meta ULC. All rights reserved