Mesenchymal stem cells enhance lung recovery after injury, shock, and chronic stress

Surgery
Amy V GoreZiad C Sifri

Abstract

Normal lung healing is impaired when lung contusion (LC) is followed by hemorrhagic shock (HS), and chronic stress (CS). Mesenchymal stem cells (MSCs) are immunomodulatory, pluripotent cells that are under investigation for use in wound healing and tissue regeneration. We hypothesized that treatment with MSCs can reverse the impaired healing seen after LC combined with HS and CS (LCHS/CS). Male Sprague-Dawley rats (n = 6/group) underwent LCHS with or without a single intravenous dose of 5 × 10(6) Sprague-Dawley rat MSCs after resuscitation. Thereafter, rats were subjected to 2 hours of CS daily on days 1-6 and were humanely killed on day 7. Lung histology was scored according to a well-established lung injury score (LIS) that included interstitial and pulmonary edema, alveolar integrity, and inflammatory cells. Scoring ranges from 0 (normal lung) to 11 (most severely injured). Whole blood was analyzed for the presence of CD4(+)CD25(+)FoxP3(+) T-regulatory cells (Treg) by flow cytometry. Seven days after isolated LC, LIS had returned to 0.8 ± 0.4; however, after LCHS/CS healing is significantly delayed (7.2 ± 2.2; P < .05). Addition of MSC to LCHS/CS decreased LIS to 2.0 ± 1.3 (P < .05) and decreased all subgroup scores (inflamm...Continue Reading

References

Oct 1, 1976·The British Journal of Surgery·F P McGinn
Jan 1, 1966·Archives of Surgery·D J GuineyG A Donaldson
Nov 4, 1995·Lancet·J K Kiecolt-GlaserR Glaser
Apr 1, 1995·The Surgical Clinics of North America·F A Moore, E E Moore
Jun 13, 1998·Psychosomatic Medicine·P T MaruchaM Favagehi
Nov 27, 1998·The American Psychologist·J K Kiecolt-GlaserR Glaser
Jul 18, 2000·The Journal of Surgical Research·J A ClaridgeJ S Young
Jul 24, 2001·Langenbeck's Archives of Surgery·C Hierholzer, T R Billiar
Jun 29, 2004·Psychoneuroendocrinology·Courtney E DetillionA Courtney DeVries
Dec 7, 2005·Archives of General Psychiatry·Janice K Kiecolt-GlaserRonald Glaser
Apr 4, 2006·Nature Medicine·Yoshinori MiyaharaHidezo Mori
Mar 9, 2007·Brain, Behavior, and Immunity·Niels EijkelkampPhillip T Marucha
Apr 10, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jianguo XuMauricio Rojas
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jonathan P Godbout, Ronald Glaser
May 13, 2008·Lancet·Katarina Le BlancUNKNOWN Developmental Committee of the European Group for Blood and Marrow Transplantation
Jun 21, 2008·Nature Reviews. Immunology·Dario A A VignaliCreg J Workman
Jul 31, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabienne VenetAlfred Ayala
Jan 5, 2011·Critical Care Medicine·Michael A MatthayJae Woo Lee
Aug 10, 2011·The Journal of Trauma·Edward J HannoushDavid H Livingston
Aug 7, 2012·Surgery·Edward J HannoushDavid H Livingston
Mar 26, 2015·The Journal of Trauma and Acute Care Surgery·Amy V GoreZiad C Sifri

❮ Previous
Next ❯

Citations

Jun 25, 2016·Stem Cells International·Markus Huber-LangRolf E Brenner
Jul 13, 2016·Shock·Melissa A Kottke, Thomas J Walters
Jun 3, 2021·International Journal of Molecular Sciences·Raphael Gorodetsky, Wilhelm K Aicher
Aug 12, 2021·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Vinicius Luderer DiasPaulo Manuel Pêgo-Fernandes

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.