Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xuxin ChenZhihai Han

Abstract

Bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation has therapeutic effects on endothelial damage during acute lung injury (ALI). Heme oxygenase-1 (HO-1) can restore homeostasis and implement cytoprotective defense functions in many pathologic states. Therefore, we explored whether transduction of HO-1 into BM-MSCs (MSCs-HO-1) would have an increased beneficial effect on lipopolysaccharide (LPS)-induced inflammatory and oxidative damage in human pulmonary microvascular endothelial cells (PVECs). MSCs were isolated from rat bone marrow and transfected with the HO-1 gene by a lentivirus vector. The phenotype and multilineage differentiation of MSCs were assessed. MSCs or MSCs-HO-1 were co-cultured with PVECs using a transwell system, and LPS was added to induce PVEC injury. The production of reactive oxygen species (ROS), and the activities of lipid peroxide (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in PVECs were determined by flow cytometry and colorimetric assays, respectively. The levels of human PVEC-derived tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in the supernatants of the co-culture system, and the activity of nuclear transcription factor-...Continue Reading

Citations

Jan 23, 2019·Acta Biochimica Et Biophysica Sinica·Rui HaoGuohai Su
Jul 4, 2019·Current Gene Therapy·Xue-Peng ZhangZhe Luo
Jan 23, 2020·Cellular and Molecular Life Sciences : CMLS·Xing-Liang FanQing-Ling Fu
Oct 5, 2019·The Journal of International Medical Research·Zejia SunWei Wang
Jul 25, 2019·International Journal of Molecular Sciences·Kuo-Hua LeeDer-Cherng Tarng
Apr 16, 2020·Current Hypertension Reports·S SuvakovL McClements
Feb 7, 2020·Cell & Bioscience·Jianhan RenChen Zhou
Jul 13, 2021·World Journal of Stem Cells·Amit SharmaBithiah Grace Jaganathan

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