Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α

Stem Cells International
Chang LiuLingyun Sun

Abstract

To investigate the effects of umbilical cord mesenchymal stem cell (UC-MSC) transplantation on joint damage and osteoporosis in collagen-induced arthritis (CIA) mice and to explore the mechanisms by which UC-MSCs modulate the osteogenic differentiation. CIA mice were divided into the following treated groups: UC-MSC transplantation group, antitumor necrosis factor- (TNF-) α group, and zoledronic acid (ZA) group. Microcomputed tomography (micro-CT) was used to analyze the bone morphology parameters. Osteogenic differentiation of treated CIA mice was determined. Bone marrow mesenchymal stem cells (BM-MSCs) from CIA mice were treated with TNF-α in vitro to explore their effects on osteogenesis. The arthritis score was significantly reduced in the UC-MSC transplantation and anti-TNF-α-treated CIA groups, compared with control mice (P < 0.001). Micro-CT showed that CIA mice developed osteoporosis at 12 weeks after immunization. The bone morphology parameters were partially improved in UC-MSC-treated CIA mice. Impaired osteogenic differentiation functions were indicated by decreased ALP activity (P < 0.001) and reduced mRNA and protein levels of osteogenic marker genes (P < 0.05) in CIA mice compared with DBA/1 mice. UC-MSC treatment...Continue Reading

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Citations

Oct 29, 2019·Journal of Cellular and Molecular Medicine·Ying ZhangWuyin Li
Jul 10, 2019·Stem Cells International·Vasilis Paspaliaris, George Kolios
Jan 28, 2020·International Journal of Molecular Medicine·Qiushi WeiYanming Cao
Dec 12, 2018·Journal of Cellular and Molecular Medicine·Xinxing ZhuJuntang Lin
Aug 7, 2021·International Journal of Biological Macromolecules·Özge Karabıyık AcarGamze Torun Kose

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Methods Mentioned

BETA
Reverse Transcription PCR
PCR
ELISA

Software Mentioned

Skyscan CTAn
SkyScan CT Analyzer
GraphPad

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