Mesenchymal stromal cell-derived nanovesicles ameliorate bacterial outer membrane vesicle-induced sepsis via IL-10

Stem Cell Research & Therapy
Kyong-Su ParkJan Lötvall

Abstract

Sepsis remains a source of high mortality in hospitalized patients despite proper antibiotic approaches. Encouragingly, mesenchymal stromal cells (MSCs) and their produced extracellular vesicles (EVs) have been shown to elicit anti-inflammatory effects in multiple inflammatory conditions including sepsis. However, EVs are generally released from mammalian cells in relatively low amounts, and high-yield isolation of EVs is still challenging due to a complicated procedure. To get over these limitations, vesicles very similar to EVs can be produced by serial extrusions of cells, after which they are called nanovesicles (NVs). We hypothesized that MSC-derived NVs can attenuate the cytokine storm induced by bacterial outer membrane vesicles (OMVs) in mice, and we aimed to elucidate the mechanism involved. NVs were produced from MSCs by the breakdown of cells through serial extrusions and were subsequently floated in a density gradient. Morphology and the number of NVs were analyzed by transmission electron microscopy and nanoparticle tracking analysis. Mice were intraperitoneally injected with Escherichia coli-derived OMVs to establish sepsis, and then injected with 2 × 109 NVs. Innate inflammation was assessed in peritoneal fluid a...Continue Reading

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Citations

Aug 17, 2020·Stem Cell Research & Therapy·Ali Akbari, Jafar Rezaie
Jul 3, 2020·International Journal of Molecular Sciences·Katarzyna Nazimek, Krzysztof Bryniarski
Aug 28, 2020·International Journal of Molecular Sciences·Simona VillataValentina Cauda
Jul 14, 2020·Frontiers in Veterinary Science·Metka VogaGregor Majdic
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Yiwei FengMei Cui
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Andrea PapaitOrnella Parolini
Nov 8, 2020·International Journal of Molecular Sciences·Hector GonzalezDaniel O'Toole
Dec 18, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Maja SevericWafa T Al-Jamal
Mar 11, 2020·Biomaterials·Mei Lu, Yuanyu Huang
Aug 24, 2021·Advanced Therapeutics·Stephanie M KronstadtSteven M Jay
Sep 3, 2021·Stem Cells International·Can JinHui Qian

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Methods Mentioned

BETA
transmission electron microscopy
electron microscopy
Fusion Tribrid
Flow cytometry
bronchoalveolar lavage
flow
light microscopy
ELISA
profiler
PMA

Software Mentioned

Percolator
Mascot
DAVID
FlowJo
BD FACSuit
ZetaView
Funrich
Panther
Living Image
Proteome Discoverer

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