Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress

Stem Cells International
Timo Z Nazari-ShaftiChristof Stamm

Abstract

Despite regulatory issues surrounding the use of animal-derived cell culture supplements, most clinical cardiac cell therapy trials using mesenchymal stromal cells (MSCs) still rely on fetal bovine serum (FBS) for cell expansion before transplantation. We sought to investigate the effect of human serum from heart failure patients (HFS) on cord blood MSCs (CB-MSCs) during short-term culture under regular conditions and during simulated acute and chronic stress. Cell survival, proliferation, metabolic activity, and apoptosis were quantified, and gene expression profiles of selected apoptosis and cell cycle regulators were determined. Compared to FBS, HFS and serum from healthy donors (CS) showed similar effects by substantially increasing cell survival during chronic and acute stress and by increasing cell yields 5 days after acute stress. Shortly after the termination of acute stress, both HFS and CS resulted in a marked decrease in apoptotic cells. Transcriptome analysis suggested a decrease in TNF-mediated induction of caspases and decreased activation of mitochondrial apoptosis. Our data confirm that human serum from both healthy donors and heart failure patients results in increased cell yields and increased resistance to ce...Continue Reading

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Methods Mentioned

BETA
Protein Assay
ELISA
electrophoresis
flow cytometry
PCR

Clinical Trials Mentioned

NCT01768702
NCT00810238
NCT02323477
NCT00721045
NCT00644410
NCT00883727
NCT01392105
NCT01291329
NCT00768066
NCT00587990

Software Mentioned

IMPACT
CURE
Columbus
PerkinElmer
SPSS
DCM
CHART

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