Mesenchymal Stromal Cells Differentiating to Adipocytes Accumulate Autophagic Vesicles Instead of Functional Lipid Droplets

Journal of Cellular Physiology
Alexandra Teodora GruiaFelix Aurel Mic

Abstract

Adult bone marrow mesenchymal stromal cells (BMSCs) can easily be differentiated into a variety of cells. In vivo transplantation of BMSCs-differentiated cells has had limited success, suggesting that these cells may not be fully compatible with the cells they are intended to replace in vivo. We investigated the structural and functional features of BMSCs-derived adipocytes as compared with adipocytes from adipose tissue, and the structure and functionality of lipid vesicles formed during BMSCs differentiation to adipocytes. Gas chromatography-mass spectrometry showed fatty acid composition of BMSCs-derived adipocytes and adipocytes from the adipose tissue to be very different, as is the lipid rafts composition, caveolin-1 expression, caveolae distribution in their membranes, and the pattern of expression of fatty acid elongases. Confocal microscopy confirmed the absence from BMSCs-derived adipocytes of markers of lipid droplets. BMSCs-derived adipocytes cannot convert deuterated glucose into deuterated species of fatty acids and cannot uptake the deuterated fatty acid-bovine serum albumin complexes from the culture medium, suggesting that intra-cellular accumulation of lipids does not occur by lipogenesis. We noted that BMSCs ...Continue Reading

References

Mar 1, 1991·American Journal of Obstetrics and Gynecology·Y P WangA P Killam
Jun 9, 2001·Experimental Biology and Medicine·J J MinguellP Conget
Aug 24, 2002·Science·Raymond F CastroH David Shine
Oct 1, 2003·Molecular Biology of the Cell·Hans ThornPeter Stralfors
Mar 23, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Seh-Hoon OhBryon E Petersen
Feb 26, 2005·The Journal of Biological Chemistry·Nathan E WolinsPerry E Bickel
Feb 17, 2006·Journal of Cellular Physiology·Danielle L MackayStephen E Haynesworth
Apr 29, 2006·Lab Animal·Valentin L OrdodiVirgil Paunescu
Jun 23, 2006·Journal of Lipid Research·Yun WangDonald B Jump
Mar 17, 2009·Biochemical and Biophysical Research Communications·Masahiro ShibataYasuo Uchiyama
Apr 3, 2009·Nature·Rajat SinghMark J Czaja
May 16, 2009·Molecular Endocrinology·Chong XuGuoheng Xu
May 20, 2009·The Journal of Cell Biology·Anna V BulankinaStefan Höning
Sep 19, 2009·Methods in Molecular Biology·Donald B Jump
Nov 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhangShengkan Jin
Jan 30, 2010·Autophagy·Scott GoldmanShengkan Jin
May 13, 2010·BMC Developmental Biology·Shu-Wen QianQi-Qun Tang
Oct 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qiang ChenChundong Yu
Nov 5, 2010·The Journal of Clinical Endocrinology and Metabolism·Julia KovsanAssaf Rudich
Mar 23, 2011·Trends in Endocrinology and Metabolism : TEM·Hanqing Dong, Mark J Czaja
Jun 1, 2011·Histochemistry and Cell Biology·Alexandra T GruiaFelix A Mic
Aug 19, 2011·Arthritis Research & Therapy·Chao ShenLi-Yang Dai
Apr 24, 2012·Stem Cells and Development·Lisa OliverFrançois M Vallette
Apr 27, 2012·International Journal of Cell Biology·Rajat Singh, Ana Maria Cuervo
Sep 29, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yong ZhangShengkan Jin

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Citations

Jun 17, 2021·Stem Cell Reviews and Reports·Daniela S C BispoAna M Gil

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