Mesenchymal stromal cells enhance the engraftment of hematopoietic stem cells in an autologous mouse transplantation model

Stem Cell Research & Therapy
María Fernández-GarcíaMaría Luisa Lamana

Abstract

Studies have proposed that mesenchymal stem cells (MSCs) improve the hematopoietic engraftment in allogeneic or xenogeneic transplants and this is probably due to the MSCs' immunosuppressive properties. Our study aimed to discern, for the first time, whether MSC infusion could facilitate the engraftment of hematopoietic stem cells (HSCs) in autologous transplantations models, where no immune rejection of donor HSCs is expected. Recipient mice (CD45.2) mice, conditioned with moderate doses of radiation (5-7 Gy), were transplanted with low numbers of HSCs (CD45.1/CD45.2) either as a sole population or co-infused with increasing numbers of adipose-derived-MSCs (Ad-MSCs). The influence of Ad-MSC infusion on the short-term and long-term engraftment of donor HSCs was investigated. Additionally, homing assays and studies related with the administration route and with the Ad-MSC/HSC interaction were conducted. Our data show that the co-infusion of Ad-MSCs with low numbers of purified HSCs significantly improves the short-term and long-term hematopoietic reconstitution of recipients conditioned with moderate irradiation doses. This effect was Ad-MSC dose-dependent and associated with an increased homing of transplanted HSCs in recipient...Continue Reading

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Citations

Mar 30, 2016·World Journal of Stem Cells·Ann De Becker, Ivan Van Riet
Jan 16, 2018·Stem Cells International·Vivian Fonseca GonzagaIrina Kerkis
Jul 28, 2019·Experimental Dermatology·Shadi KhalilArturo P Saavedra
Feb 6, 2018·Advanced Pharmaceutical Bulletin·Hamze TimariSara Aqmasheh
Jan 1, 2017·Cell Biology and Toxicology·Bárbara Cristina Martins Fernandes PaesVirginia Picanço-Castro
Aug 19, 2017·Human Gene Therapy Methods·María Fernández-GarcíaRosa Yañez
Mar 13, 2020·Cellular & Molecular Biology Letters·Jing ZhangXingbin Hu
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Nov 25, 2020·Blood Advances·Andre J BurnhamEdwin M Horwitz
Mar 12, 2021·Frontiers in Immunology·Tijana MartinovAnthony Rongvaux
May 13, 2021·Health Science Reports·Mohammad RafieeMohammad Hossein Mohammadi
Apr 8, 2021·Radiation Research·Ashley R Sweeney-AmbrosMegan E Oest
May 22, 2021·Frontiers in Cell and Developmental Biology·Stefania CrippaMaria Ester Bernardo
Jul 17, 2021·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Ankur JainSubhash Varma
Nov 19, 2021·Journal of Hematology & Oncology·Tianxia LanXiawei Wei

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Datasets Mentioned

BETA
ES280790000183

Methods Mentioned

BETA
flow cytometry

Software Mentioned

FlowJo
FlowJo Analysis ( Tree Star
GraphPad
GraphPad Prism

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