Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro

Theranostics
Lucienne A VonkMagdalena J Lorenowicz

Abstract

Osteoarthritis (OA) is a rheumatic disease leading to chronic pain and disability with no effective treatment available. Recently, allogeneic human mesenchymal stromal/stem cells (MSC) entered clinical trials as a novel therapy for OA. Increasing evidence suggests that therapeutic efficacy of MSC depends on paracrine signalling. Here we investigated the role of extracellular vesicles (EVs) secreted by human bone marrow derived MSC (BMMSC) in human OA cartilage repair. To test the effect of BMMSC-EVs on OA cartilage inflammation, TNF-alpha-stimulated OA chondrocyte monolayer cultures were treated with BMMSC-EVs and pro-inflammatory gene expression was measured by qRT-PCR after 48 h. To assess the impact of BMMSC-EVs on cartilage regeneration, BMMSC-EVs were added to the regeneration cultures of human OA chondrocytes, which were analyzed after 4 weeks for glycosaminoglycan content by 1,9-dimethylmethylene blue (DMMB) assay. Furthermore, paraffin sections of the regenerated tissue were stained for proteoglycans (safranin-O) and type II collagen (immunostaining). We show that BMMSC-EVs inhibit the adverse effects of inflammatory mediators on cartilage homeostasis. When co-cultured with OA chondrocytes, BMMSC-EVs abrogated the TNF-a...Continue Reading

Citations

Nov 1, 2018·Journal of Biomedical Materials Research. Part a·Chenyu ChuYili Qu
Nov 7, 2018·Tissue Engineering. Part C, Methods·Michella H HagmeijerDaniel B F Saris
Oct 20, 2018·Journal of Cellular Biochemistry·Tingshuai Zhang, Yongchang Yao
Feb 8, 2019·Current Pharmaceutical Biotechnology·Marta A SzychlinskaGiuseppe Musumeci
Nov 30, 2019·Current Pharmaceutical Design·Su-Kang ShanLing-Qing Yuan
Mar 25, 2020·Cell Transplantation·Bocheng ZhangWeiguo Zhang
May 5, 2020·Cellular Reprogramming·Jian WangShane Gao
May 31, 2020·International Journal of Molecular Sciences·Irene C MarcuJasmin Bluteau
Aug 12, 2020·Basic & Clinical Pharmacology & Toxicology·Xiaoqin Wang, Peter Thomsen
Feb 17, 2019·Nanomaterials·Jiao Jiao LiChristopher B Little
Jul 1, 2020·Cellular and Molecular Life Sciences : CMLS·Abazar EsmaeiliMohamadreza Baghaban Eslaminejad
Apr 26, 2019·Cell Transplantation·Aleksandra Musiał-WysockaMarcin Majka
Dec 6, 2019·Frontiers in Bioengineering and Biotechnology·Kendrick ToWasim Khan
Feb 18, 2020·Journal of Tissue Engineering and Regenerative Medicine·Archna DhasmanaSagar Rawal
Nov 7, 2019·Journal of Clinical Medicine·Daniele D'ArrigoGiuseppe Filardo
Jan 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Minwook KimRobert L Mauck
Nov 23, 2019·Stem Cell Research & Therapy·Elaheh MianehsazMichael R Hamblin
May 5, 2020·Frontiers in Bioengineering and Biotechnology·Nathan PiazzaKarin Wuertz-Kozak
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Maria Elisabetta Federica PalamàChiara Gentili
Mar 7, 2020·Frontiers in Cell and Developmental Biology·Riam ShammaaMoutih Rafei
Nov 30, 2018·Frontiers in Immunology·Janneke BoereMarca H M Wauben
Oct 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xingyu ZhangShiyi Chen

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Methods Mentioned

BETA
density gradient centrifugation
DNA assay
PCR
density gradient purification
confocal microscopy
nuclear translocation
electron microscopy

Clinical Trials Mentioned

NCT02037204

Software Mentioned

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