Meso-Endothelial Bipotent Progenitors from Human Placenta Display Distinct Molecular and Cellular Identity

Stem Cell Reports
Abbas ShafieeKiarash Khosrotehrani

Abstract

The existence of bipotential precursors for both mesenchymal and endothelial stem/progenitor cells in human postnatal life is debated. Here, we hypothesized that such progenitors are present within the human term placenta. From a heterogeneous placental single-cell suspension, a directly flow-sorted CD45-CD34+CD144+CD31Lo population uniquely differentiated into both endothelial and mesenchymal colonies in limiting dilution culture assays. Of interest, these bipotent cells were in vessel walls but not in contact with the circulation. RNA sequencing and functional analysis demonstrated that Notch signaling was a key driver for endothelial and bipotential progenitor function. In contrast, the formation of mesenchymal cells from the bipotential population was not affected by TGFβ receptor inhibition, a classical pathway for endothelial-mesenchymal transition. This study reveals a bipotent progenitor phenotype in the human placenta at the cellular and molecular levels, giving rise to endothelial and mesenchymal cells ex vivo.

Citations

Sep 27, 2018·Human Reproduction Update·Anna L BossJoanna L James
May 3, 2019·Cellular and Molecular Life Sciences : CMLS·Martin KnöflerJoanna James
Aug 28, 2021·Frontiers in Cell and Developmental Biology·Christopher StemGraça Almeida-Porada
Nov 2, 2021·Stem Cells Translational Medicine·Sarah E J ChambersReinhold J Medina

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP127770

Methods Mentioned

BETA
Flow cytometry
magnetic activated cell sorting
PCR
Assay
Flow-cytometry
fluorescence-activated cell sorting

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Related Papers

International Journal of Medical Sciences
Wei-Xiu Zhao, Jian-Hua Lin
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
Xiaoying ZouXuejun Gao
© 2022 Meta ULC. All rights reserved