Mesoporous carbon-enriched palladium nanostructures with redox activity for enzyme-free electrochemical immunoassay of brevetoxin B

Analytica Chimica Acta
Youxiu LinDianping Tang

Abstract

A new signal amplification strategy based on mesoporous carbon-enriched palladium nanostructure (MSC-PdNS) was designed for enzyme-free electrochemical immunoassay of brevetoxin B (BTB) in marine toxins. The assay was carried out on a BTB-bovine serum albumin-functionalized electrode by using monoclonal mouse anti-BTB-labeling MSC-PdNS as the signal-transduction tag. A competitive-type assay protocol was successfully introduced to develop a high-efficiency enzyme-free immunoassay accompanying the doped palladium nanostructure into MSC-PdNS toward reduction of H2O2. Under the optimal conditions, the catalytic current decreased with the increment of BTB concentration in the range from 0.01 to 10 ng mL(-1) with a detection limit (LOD) of 5.0 pg mL(-1) BTB at the 3s(blank) criterion. The selectivity and precision were acceptable. In addition, the methodology was further validated for assaying spiked seafood samples, and consistent results between the electrochemical immunoassay and the referenced enzyme immunoassay were obtained. Importantly, the enzyme-free electrochemical immunoassay provides a promising approach for rapid screening of marine toxin because of its simplicity, low cost, sensitivity, specificity and without the need...Continue Reading

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Citations

Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Feb 23, 2021·Journal of Drug Targeting·Mohammad Reza KhirehgeshBahman Akbari
Feb 7, 2016·Biosensors & Bioelectronics·Wenqiang LaiDianping Tang
Jul 4, 2021·Talanta·Saipriya RamalingamAshutosh Singh
Jan 21, 2017·Analytical Chemistry·Wei WenYuehe Lin

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