Mesoporous Silica Nanoparticles Rescue H2 O2 -induced Inhibition of Cardiac Differentiation

Cell Structure and Function
Mingming RenZhen Han

Abstract

The anti-oxidative property of mesoporous silica nanoparticles (MSNs) has been proposed previously, which prompted us to investigate the potential protective effect of MSNs on human embryonic stem cells (hESCs) against oxidative stress. To this purpose, the cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Apoptosis was analyzed by Annexin V/propidium iodide double-staining method. The intracellular glutathione, superoxide dismutase and malondialdehyde were measured with commercial assay kits. The reactive oxygen species was detected by staining with fluorescent dye DCFH-DA. The relative levels of Nkx2.5, Mef2c, Tbx5, dHand and α-MHC transcripts were measured by real-time polymerase chain reaction. The protein levels of Connexin 43, Troponin C1 and GAPDH were determined by immunoblotting. The beating behavior of embryoid bodies (EBs) was visually examined. Our results demonstrated that MSNs reversed hydrogen peroxide (H2O2)-inhibited cell viability and ameliorated H2O2-induced cell apoptosis in vitro. The H2O2-elicited intracellular oxidative stress was significantly relieved in the presence of MSNs. Furthermore, MSNs improved H2O2-suppressed differentiation of hESC-derive...Continue Reading

References

Sep 25, 2003·Cell·Antonio P BeltramiPiero Anversa
Apr 9, 2008·Biomaterials·Eric G HeckertWilliam T Self
Mar 20, 2012·Cell·Sekar Kathiresan, Deepak Srivastava
Jan 8, 2013·Journal of the American Chemical Society·Yi-Ping ChenChung-Yuan Mou
Jun 12, 2013·Cell Stem Cell·Jessica C Garbern, Richard T Lee
Dec 3, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Ying WangSiling Wang
Dec 19, 2014·Circulation·Dariush MozaffarianUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Jun 27, 2015·ACS Nano·Samir MitragotriChenjie Xu
Jul 15, 2016·Oxidative Medicine and Cellular Longevity·Ryan A Denu, Peiman Hematti

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis