Mesoscopic Energy Minimization Drives Pseudomonas aeruginosa Biofilm Morphologies and Consequent Stratification of Antibiotic Activity Based on Cell Metabolism

Antimicrobial Agents and Chemotherapy
M V SheratonP M A Sloot

Abstract

Segregation of bacteria based on their metabolic activities in biofilms plays an important role in the development of antibiotic resistance. Mushroom-shaped biofilm structures, which are reported for many bacteria, exhibit topographically varying levels of multiple drug resistance from the cap of the mushroom to its stalk. Understanding the dynamics behind the formation of such structures can aid in design of drug delivery systems, antibiotics, or physical systems for removal of biofilms. We explored the development of metabolically heterogeneous Pseudomonas aeruginosa biofilms using numerical models and laboratory knockout experiments on wild-type and chemotaxis-deficient mutants. We show that chemotactic processes dominate the transformation of slender and hemispherical structures into mushroom structures with a signature cap. Cellular Potts model simulation and experimental data provide evidence that accelerated movement of bacteria along the periphery of the biofilm, due to nutrient cues, results in the formation of mushroom structures and bacterial segregation. Multidrug resistance of bacteria is one of the most threatening dangers to public health. Understanding the mechanisms of the development of mushroom-shaped biofilm...Continue Reading

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Citations

Mar 27, 2019·Physical Biology·Vernita GordonKristin Kovach
Dec 11, 2019·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim
Jun 20, 2020·The ISME Journal·Liam M RooneyGail McConnell

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Methods Mentioned

BETA
chemical treatments
confocal microscopy

Software Mentioned

IMARIS

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