Messenger RNA and protein expression analysis of betaglycan in the pituitary and ovary of the domestic hen

Biology of Reproduction
Sheila A Sweeney, Patricia A Johnson

Abstract

Betaglycan was originally characterized as the type III receptor for TGFbeta, yet recent research has indicated that betaglycan can serve as an accessory receptor for inhibin. To understand better the action of inhibin in avian follicular development, we have investigated the expression of betaglycan in the pituitary gland and ovary of the hen. In experiments 1 and 2, betaglycan mRNA was detected at 6 kilobases (kb) by Northern blot analysis (n = 5) in chicken pituitary, granulosa, and theca layers and whole ovary. Expression of betaglycan was greatest in the pituitary gland in experiment 1 and greater in the granulosa layer of small yellow follicles (SYF) compared with the granulosa layer of larger follicles. In experiment 2, betaglycan mRNA was more abundantly expressed in the theca layer compared with the granulosa layer for all follicle sizes, although there was no significant difference in betaglycan expression in the theca layer among follicle sizes. In experiment 3, immunohistochemical analysis revealed betaglycan protein in the anterior pituitary as well as in the ovary (n = 4) and SYF (n = 4). Colocalization studies revealed a high abundance of cells within the anterior pituitary expressing both betaglycan and FSH (n =...Continue Reading

References

Nov 30, 1992·Biochemical and Biophysical Research Communications·A MorénK Miyazono
Dec 1, 1992·Biology of Reproduction·D VanmontfortG Verhoeven
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J HsuehW Vale
Jun 1, 1995·Endocrinology·B Le Magueresse-BattistoniM Benahmed
Mar 17, 1995·The Journal of Biological Chemistry·J XuW L McKeehan
Jan 1, 1994·Reproduction, Fertility, and Development·J K Findlay
Jan 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J V BarnettR L Maas
Aug 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R H SchwallT G Terrell
Jan 1, 1996·Proceedings of the Society for Experimental Biology and Medicine·T MurataK Torii
Mar 1, 1996·Fertility and Sterility·L M Halvorson, A H DeCherney
Jul 1, 1997·Proceedings of the Society for Experimental Biology and Medicine·J P MatherR H Li
Mar 10, 2001·Recent Progress in Hormone Research·D J BernardT K Woodruff
Mar 27, 2001·Molecular Endocrinology·S C Chapman, T K Woodruff
Jan 31, 2002·Molecular Endocrinology·Daniel J BernardTeresa K Woodruff

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Citations

Apr 5, 2005·General and Comparative Endocrinology·P A JohnsonA J Davis
Jul 17, 2013·Reproductive Biology and Endocrinology : RB&E·Qingming QinZhendan Shi
Apr 25, 2009·Journal of Foot and Ankle Research·Christian J BartonHylton B Menz
Nov 26, 2008·Animal Reproduction Science·Okanlawon OnagbesanEddy Decuypere

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Methods Mentioned

BETA
dissection
PCR

Software Mentioned

Scion Image densitometry
Primer Design

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